1hys: Difference between revisions

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[[Image:1hys.gif|left|200px]]
[[Image:1hys.gif|left|200px]]


{{Structure
<!--
|PDB= 1hys |SIZE=350|CAPTION= <scene name='initialview01'>1hys</scene>, resolution 3.0&Aring;
The line below this paragraph, containing "STRUCTURE_1hys", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span>
or leave the SCENE parameter empty for the default display.
|GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
-->
|DOMAIN=
{{STRUCTURE_1hys|  PDB=1hys |  SCENE= }}  
|RELATEDENTRY=[[2hmi|2HMI]], [[1rtd|1RTD]], [[1dlo|1DLO]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hys OCA], [http://www.ebi.ac.uk/pdbsum/1hys PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hys RCSB]</span>
}}


'''CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA'''
'''CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA'''
Line 36: Line 33:
[[Category: Tantillo, C.]]
[[Category: Tantillo, C.]]
[[Category: Whitcomb, J.]]
[[Category: Whitcomb, J.]]
[[Category: polypurine tract]]
[[Category: Polypurine tract]]
[[Category: ppt]]
[[Category: Ppt]]
[[Category: protein-nucleic acid complex]]
[[Category: Protein-nucleic acid complex]]
[[Category: rna:dna]]
[[Category: Rna:dna]]
[[Category: rnase h]]
[[Category: Rnase h]]
[[Category: rnase h primer grip]]
[[Category: Rnase h primer grip]]
[[Category: unpaired base]]
[[Category: Unpaired base]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:22:21 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:12:14 2008''

Revision as of 16:22, 2 May 2008

File:1hys.gif

Template:STRUCTURE 1hys

CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE IN COMPLEX WITH A POLYPURINE TRACT RNA:DNA


Overview

We have determined the 3.0 A resolution structure of wild-type HIV-1 reverse transcriptase in complex with an RNA:DNA oligonucleotide whose sequence includes a purine-rich segment from the HIV-1 genome called the polypurine tract (PPT). The PPT is resistant to ribonuclease H (RNase H) cleavage and is used as a primer for second DNA strand synthesis. The 'RNase H primer grip', consisting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis and cleavage specificity. Cleavage specificity is also controlled by the width of the minor groove and the trajectory of the RNA:DNA, both of which are sequence dependent. An unusual 'unzipping' of 7 bp occurs in the adenine stretch of the PPT: an unpaired base on the template strand takes the base pairing out of register and then, following two offset base pairs, an unpaired base on the primer strand re-establishes the normal register. The structural aberration extends to the RNase H active site and may play a role in the resistance of PPT to RNase H cleavage.

About this Structure

1HYS is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA., Sarafianos SG, Das K, Tantillo C, Clark AD Jr, Ding J, Whitcomb JM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2001 Mar 15;20(6):1449-61. PMID:11250910 Page seeded by OCA on Fri May 2 19:22:21 2008

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