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| ==Solution Structure of ERCC4 domain of human FAAP24== | | ==Solution Structure of ERCC4 domain of human FAAP24== |
| <StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | | <StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2m9n|2m9n]]</div></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[https://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The FANCM/FAAP24 heterodimer has distinct functions in protecting cells from complex DNA lesions such as interstrand crosslinks. These functions rely on the biochemical activity of FANCM/FAAP24 to recognize and bind to damaged DNA or stalled replication forks. However, the DNA-binding activity of this complex was not clearly defined. We investigated how FAAP24 contributes to the DNA-interacting functions of the FANCM/FAAP24 complex by acquiring the N-terminal and C-terminal solution structures of human FAAP24. Modeling of the FAAP24 structure indicates that FAAP24 may possess a high affinity toward single-stranded DNA (ssDNA). Testing of various FAAP24 mutations in vitro and in vivo validated this prediction derived from structural analyses. We found that the DNA-binding and FANCM-interacting functions of FAAP24, although both require the C-terminal (HhH)2 domain, can be distinguished by segregation-of-function mutations. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through the formation of FANCM/FAAP24 heterodimer and the other via its ssDNA-binding activity required in optimized checkpoint activation.Cell Research advance online publication 3 September 2013; doi:10.1038/cr.2013.124.
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| Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response.,Wang Y, Han X, Wu F, Leung JW, Lowery MG, Do H, Chen J, Shi C, Tian C, Li L, Gong W Cell Res. 2013 Sep 3. doi: 10.1038/cr.2013.124. PMID:23999858<ref>PMID:23999858</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2m9m" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Gong, W]] | | [[Category: Gong W]] |
| [[Category: Han, X]] | | [[Category: Han X]] |
| [[Category: Shi, C]] | | [[Category: Shi C]] |
| [[Category: Tian, C]] | | [[Category: Tian C]] |
| [[Category: Wu, F]] | | [[Category: Wu F]] |
| [[Category: Dna binding protein]]
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| [[Category: Ercc4 domain]]
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| [[Category: Faap24]]
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| [[Category: Fanconi anemia]]
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