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| ==Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode== | | ==Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode== |
| <StructureSection load='4ou7' size='340' side='right'caption='[[4ou7]], [[Resolution|resolution]] 2.83Å' scene=''> | | <StructureSection load='4ou7' size='340' side='right'caption='[[4ou7]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4ou7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OU7 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OU7 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ou6|4ou6]]</div></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ou7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou7 OCA], [https://pdbe.org/4ou7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ou7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ou7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou7 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ou7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou7 OCA], [https://pdbe.org/4ou7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ou7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ou7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou7 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function ==
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| [[https://www.uniprot.org/uniprot/DNAT_ECOLI DNAT_ECOLI]] This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.[HAMAP-Rule:MF_01061]
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| DnaT is a primosomal protein that is required for the stalled replication fork restart in Escherichia coli. As an adapter, DnaT mediates the PriA-PriB-ssDNA ternary complex and the DnaB/C complex. However, the fundamental function of DnaT during PriA-dependent primosome assembly is still a black box. Here, we report the 2.83 A DnaT84-153-dT10 ssDNA complex structure, which reveals a novel three-helix bundle single-stranded DNA binding mode. Based on binding assays and negative-staining electron microscopy results, we found that DnaT can bind to phiX 174 ssDNA to form nucleoprotein filaments for the first time, which indicates that DnaT might function as a scaffold protein during the PriA-dependent primosome assembly. In combination with biochemical analysis, we propose a cooperative mechanism for the binding of DnaT to ssDNA and a possible model for the assembly of PriA-PriB-ssDNA-DnaT complex that sheds light on the function of DnaT during the primosome assembly and stalled replication fork restart. This report presents the first structure of the DnaT C-terminal complex with ssDNA and a novel model that explains the interactions between the three-helix bundle and ssDNA.
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| Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.,Liu Z, Chen P, Wang X, Cai G, Niu L, Teng M, Li X Nucleic Acids Res. 2014 Jul 22. pii: gku633. PMID:25053836<ref>PMID:25053836</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4ou7" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Chen, P]] | | [[Category: Chen P]] |
| [[Category: Li, X]] | | [[Category: Li X]] |
| [[Category: Liu, Z]] | | [[Category: Liu Z]] |
| [[Category: Niu, L]] | | [[Category: Niu L]] |
| [[Category: Teng, M]] | | [[Category: Teng M]] |
| [[Category: Dna binding]]
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| [[Category: Replication-dna complex]]
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