7phf: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 7phf is ~~ON HOLD~~

==Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK==

~~Authors~~: ~~Dinis~~, P., ~~MetsaKetela~~, M.

== Structural highlights ==

<table><tr><td colspan='2'>[[7phf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius], [https://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens] and [https://en.wikipedia.org/wiki/Streptomyces_tsukubensis_NRRL18488 Streptomyces tsukubensis NRRL18488]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHF FirstGlance]. <br>

~~Description~~: ~~Chimeric~~ carminomycin-4-O-~~methyltransferase~~ (~~DnrK) with regions from~~ 10-~~hydroxylase RdmB~~ and 10-~~decarboxylase TamK~~

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=VAK:METHYL+(1R,2R,4S)-2-ETHYL-2,4,5,7-TETRAHYDROXY-6,11-DIOXO-1,2,3,4,6,11-HEXAHYDROTETRACENE-1-CARBOXYLATE'>VAK</scene></td></tr>

[[Category: ~~Unreleased~~ Structures]]

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phf OCA], [https://pdbe.org/7phf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phf RCSB], [https://www.ebi.ac.uk/pdbsum/7phf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phf ProSAT]</span></td></tr>

[[Category: Dinis, P]]

</table>

[[Category: ~~Metsaketela,~~ M]]

== Function ==

[[https://www.uniprot.org/uniprot/I2N5E8_STRT9 I2N5E8_STRT9]] [[https://www.uniprot.org/uniprot/RDMB_STREF RDMB_STREF]] Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. In vivo and in vitro, RdmB catalyzes the removal of the carboxylic group from the C-10 position of 15-demethoxyaclacinomycin T coupled to hydroxylation at the same C-10 position. It could also catalyze the removal of the carboxylic group at the C-10 position of 15-demethoxy-epsilon-rhodomycin coupled to hydroxylation at the same C-10 position to yield rhodomycin B. The reaction catalyzes by RdmB is intriguing, since the enzyme does not use any of the cofactors usually associated with hydroxylases such as flavins and/or metal ions to activate molecular oxygen.<ref>PMID:11004563</ref> <ref>PMID:15548527</ref> [[https://www.uniprot.org/uniprot/DNRK_STRPE DNRK_STRPE]] Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.<ref>PMID:15273252</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Streptomyces peucetius]]

[[Category: Streptomyces purpurascens]]

[[Category: Streptomyces tsukubensis NRRL18488]]

[[Category: Dinis P]]

[[Category: MetsaKetela M]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 7phf is ON HOLD
+==Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK==
+<StructureSection load='7phf' size='340' side='right'caption='[[7phf]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
-Authors: Dinis, P., MetsaKetela, M.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[7phf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius], [https://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens] and [https://en.wikipedia.org/wiki/Streptomyces_tsukubensis_NRRL18488 Streptomyces tsukubensis NRRL18488]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PHF FirstGlance]. <br>
-Description: Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=VAK:METHYL+(1R,2R,4S)-2-ETHYL-2,4,5,7-TETRAHYDROXY-6,11-DIOXO-1,2,3,4,6,11-HEXAHYDROTETRACENE-1-CARBOXYLATE'>VAK</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7phf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7phf OCA], [https://pdbe.org/7phf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7phf RCSB], [https://www.ebi.ac.uk/pdbsum/7phf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7phf ProSAT]</span></td></tr>
-[[Category: Dinis, P]]
+</table>
-[[Category: Metsaketela, M]]
+== Function ==
+[[https://www.uniprot.org/uniprot/I2N5E8_STRT9 I2N5E8_STRT9]] [[https://www.uniprot.org/uniprot/RDMB_STREF RDMB_STREF]] Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. In vivo and in vitro, RdmB catalyzes the removal of the carboxylic group from the C-10 position of 15-demethoxyaclacinomycin T coupled to hydroxylation at the same C-10 position. It could also catalyze the removal of the carboxylic group at the C-10 position of 15-demethoxy-epsilon-rhodomycin coupled to hydroxylation at the same C-10 position to yield rhodomycin B. The reaction catalyzes by RdmB is intriguing, since the enzyme does not use any of the cofactors usually associated with hydroxylases such as flavins and/or metal ions to activate molecular oxygen.<ref>PMID:11004563</ref> <ref>PMID:15548527</ref> [[https://www.uniprot.org/uniprot/DNRK_STRPE DNRK_STRPE]] Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.<ref>PMID:15273252</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptomyces peucetius]]
+[[Category: Streptomyces purpurascens]]
+[[Category: Streptomyces tsukubensis NRRL18488]]
+[[Category: Dinis P]]
+[[Category: MetsaKetela M]]