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| Line 3: |
Line 3: |
| <SX load='4btq' size='340' side='right' viewer='molstar' caption='[[4btq]], [[Resolution|resolution]] 7.50Å' scene=''> | | <SX load='4btq' size='340' side='right' viewer='molstar' caption='[[4btq]], [[Resolution|resolution]] 7.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4btq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4BTQ FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4btq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BTQ FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4btg|4btg]]</td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4btq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btq OCA], [https://pdbe.org/4btq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4btq RCSB], [https://www.ebi.ac.uk/pdbsum/4btq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4btq ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4btq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4btq OCA], [http://pdbe.org/4btq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4btq RCSB], [http://www.ebi.ac.uk/pdbsum/4btq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4btq ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging. | | [[https://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging. |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The cystovirus varphi6 shares several distinct features with other double-stranded RNA (dsRNA) viruses, including the human pathogen, rotavirus: segmented genomes, nonequivalent packing of 120 subunits in its icosahedral capsid, and capsids as compartments for transcription and replication. varphi6 assembles as a dodecahedral procapsid that undergoes major conformational changes as it matures into the spherical capsid. We determined the crystal structure of the capsid protein, P1, revealing a flattened trapezoid subunit with an alpha-helical fold. We also solved the procapsid with cryo-electron microscopy to comparable resolution. Fitting the crystal structure into the procapsid disclosed substantial conformational differences between the two P1 conformers. Maturation via two intermediate states involves remodeling on a similar scale, besides huge rigid-body rotations. The capsid structure and its stepwise maturation that is coupled to sequential packaging of three RNA segments sets the cystoviruses apart from other dsRNA viruses as a dynamic molecular machine.
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| Subunit Folds and Maturation Pathway of a dsRNA Virus Capsid.,Nemecek D, Boura E, Wu W, Cheng N, Plevka P, Qiao J, Mindich L, Heymann JB, Hurley JH, Steven AC Structure. 2013 Aug 6;21(8):1374-83. doi: 10.1016/j.str.2013.06.007. Epub 2013, Jul 25. PMID:23891288<ref>PMID:23891288</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4btq" style="background-color:#fffaf0;"></div>
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| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </SX> | | </SX> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Pseudomonas phage phi6]] | | [[Category: Pseudomonas virus phi6]] |
| [[Category: Boura, E]] | | [[Category: Boura E]] |
| [[Category: Cheng, N]] | | [[Category: Cheng N]] |
| [[Category: Heymann, J B]] | | [[Category: Heymann JB]] |
| [[Category: Hurley, J H]] | | [[Category: Hurley JH]] |
| [[Category: Mindich, L]] | | [[Category: Mindich L]] |
| [[Category: Nemecek, D]] | | [[Category: Nemecek D]] |
| [[Category: Plevka, P]] | | [[Category: Plevka P]] |
| [[Category: Qiao, J]] | | [[Category: Qiao J]] |
| [[Category: Steven, A C]] | | [[Category: Steven AC]] |
| [[Category: Wu, W]] | | [[Category: Wu W]] |
| [[Category: Cystoviridae]]
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| [[Category: Flexible fitting]]
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| [[Category: Procapsid structure]]
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| [[Category: Viral protein]]
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