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| ==RRM domain from C. elegans SUP-12 bound to GGTGTGC DNA== | | ==RRM domain from C. elegans SUP-12 bound to GGTGTGC DNA== |
| <StructureSection load='4ch1' size='340' side='right'caption='[[4ch1]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | | <StructureSection load='4ch1' size='340' side='right'caption='[[4ch1]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4ch1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CH1 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4ch1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CH1 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ch0|4ch0]]</td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ch1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ch1 OCA], [https://pdbe.org/4ch1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ch1 RCSB], [https://www.ebi.ac.uk/pdbsum/4ch1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ch1 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ch1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ch1 OCA], [http://pdbe.org/4ch1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ch1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ch1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ch1 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [[https://www.uniprot.org/uniprot/H2L051_CAEEL H2L051_CAEEL]] |
| Cellular differentiation is frequently accompanied by alternative splicing, enabled by the expression of tissue-specific factors which bind to pre-mRNAs and regulate exon choice. During Caenorhabditis elegans development, muscle-specific expression of the splicing factor SUP-12, together with a member of the Fox-1 family of splicing proteins, generates a functionally distinct isoform of the fibroblast growth factor receptor EGL-15. Using a combination of NMR spectroscopy and isothermal titration calorimetry, we determined the mode of nucleic acid binding by the RNA recognition motif domain of SUP-12. The calculated structures provide the first atomic details of RNA and DNA binding by the family of proteins that include SUP-12, RBM24, RBM38/RNPC1, SEB-4 and XSeb4R. This information was further used to design strategic mutations to probe the interaction with ASD-1 and to quantitatively perturb splicing in vivo.
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| Backbone-independent nucleic acid binding by splicing factor SUP-12 reveals key aspects of molecular recognition.,Amrane S, Rebora K, Zniber I, Dupuy D, Mackereth CD Nat Commun. 2014 Sep 3;5:4595. doi: 10.1038/ncomms5595. PMID:25183497<ref>PMID:25183497</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4ch1" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Caeel]] | | [[Category: Caenorhabditis elegans]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Amrane, S]] | | [[Category: Amrane S]] |
| [[Category: Mackereth, C D]] | | [[Category: Mackereth CD]] |
| [[Category: Development]]
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| [[Category: Muscle]]
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| [[Category: Transcription-dna complex]]
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