7f6d

Reconstruction of the HerA-NurA complex from Deinococcus radiodurans

spinqualitylabelsall models 7f6d, resolution 3.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7f6d is a 8 chain structure with sequence from Deinococcus radiodurans R1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [Q9RW33_DEIRA] Publication Abstract from PubMed DNA end resection mediated by the coordinated action of nuclease and helicase is a crucial step in initiating homologous recombination. The end-resection apparatus NurA nuclease and HerA helicase are present in both archaea and bacteria. Here, we report the cryo-electron microscopy structure of a bacterial HerA-NurA complex from Deinococcus radiodurans. The structure reveals a barrel-like hexameric HerA and a distinctive NurA dimer subcomplex, which has a unique extended N-terminal region (ENR) involved in bacterial NurA dimerization and activation. In addition to the long protruding linking loop and the C-terminal alpha helix of NurA, the flexible ENR is close to the HerA-NurA interface and divides the central channel of the DrNurA dimer into two halves, suggesting a possible mechanism of DNA end processing. In summary, this work provides new insights into the structure, assembly, and activation mechanisms of bacterial DNA end resection mediated by a minimal end-resection apparatus. Mechanisms of helicase activated DNA end resection in bacteria.,Xu Y, Xu L, Qin C, Wang L, Guo J, Hua Y, Zhao Y Structure. 2022 Jul 5. pii: S0969-2126(22)00236-2. doi:, 10.1016/j.str.2022.06.005. PMID:35841886 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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