4cmm

Structure of human CD47 in complex with human Signal Regulatory Protein (SIRP) alpha v1

spinqualitylabelsall models 4cmm, resolution 1.92Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4cmm is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAG, PCA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [SHPS1_HUMAN] Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Publication Abstract from PubMed CD47 is a widely distributed membrane protein that interacts with Signal-regulatory protein alpha (SIRPalpha), an inhibitory receptor on myeloid cells that gives a do-not-eat-me signal. Manipulation of the interaction is of considerable interest in the immunotherapy of cancer and in xenotransplantation. The amino terminal ligand binding domain of SIRPalpha is highly polymorphic in contrast to the single Ig-like domain of CD47. There is confusion as to whether the polymorphisms will affect ligand binding but this is an important point for this interaction and other paired receptors being considered for as targets for therapy. We show by X-ray crystallography that one human SIRPalpha allele differing in 13 amino acid residues has a very similar binding site and that several different alleles all bind CD47 with similar affinity as expected as the residues are mostly surface exposed and distant from the binding site. A peptide from the binding site of CD47 has been reported to mimic the CD47 interaction with SIRPalpha but we could find no binding. We discuss the possible pitfalls in determining the affinity of weak interactions and also speculate on how SIRPalpha polymorphisms may have been selected by pathogens and how this may also be true in other paired receptors such as the KIRs. Polymorphisms in the human inhibitory signal-regulatory protein alpha do not affect binding to its ligand CD47.,Hatherley D, Lea SM, Johnson S, Barclay AN J Biol Chem. 2014 Feb 19. PMID:24550402 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CD47

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