8dn8: Difference between revisions

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'''Unreleased structure'''


The entry 8dn8 is ON HOLD
==CryoEM structure of the A. aeolicus WzmWzt transporter bound to 3-O-methyl-D-mannose==
<StructureSection load='8dn8' size='340' side='right'caption='[[8dn8]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8dn8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DN8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U90:3-O-methyl-alpha-D-mannopyranose'>U90</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8dn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8dn8 OCA], [https://pdbe.org/8dn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8dn8 RCSB], [https://www.ebi.ac.uk/pdbsum/8dn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8dn8 ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/O67181_AQUAE O67181_AQUAE]]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
O antigens are ubiquitous protective extensions of lipopolysaccharides in the extracellular leaflet of the Gram-negative outer membrane. Following biosynthesis in the cytosol, the lipid-linked polysaccharide is transported to the periplasm by the WzmWzt ABC transporter. Often, O antigen secretion requires the chemical modification of its elongating terminus, which the transporter recognizes via a carbohydrate-binding domain (CBD). Here, using components from A. aeolicus, we identify the O antigen structure with methylated mannose or rhamnose as its cap. Crystal and cryo electron microscopy structures reveal how WzmWzt recognizes this cap between its carbohydrate and nucleotide-binding domains in a nucleotide-free state. ATP binding induces drastic conformational changes of its CBD, terminating interactions with the O antigen. ATPase assays and site directed mutagenesis reveal reduced hydrolytic activity upon O antigen binding, likely to facilitate polymer loading into the ABC transporter. Our results elucidate critical steps in the recognition and translocation of polysaccharides by ABC transporters.


Authors: Spellmon, N., Zimmer, J.
Molecular basis for polysaccharide recognition and modulated ATP hydrolysis by the O antigen ABC transporter.,Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J Nat Commun. 2022 Sep 5;13(1):5226. doi: 10.1038/s41467-022-32597-2. PMID:36064941<ref>PMID:36064941</ref>


Description: CryoEM structure of the A. aeolicus WzmWzt transporter bound to 3-O-methyl-D-mannose
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Spellmon, N]]
<div class="pdbe-citations 8dn8" style="background-color:#fffaf0;"></div>
[[Category: Zimmer, J]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus VF5]]
[[Category: Large Structures]]
[[Category: Spellmon N]]
[[Category: Zimmer J]]
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