7xds: Difference between revisions
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==Crystal structure of wheat stem rust effector AvrSr35== | |||
<StructureSection load='7xds' size='340' side='right'caption='[[7xds]], [[Resolution|resolution]] 2.06Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7xds]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Puccinia_graminis_f._sp._tritici Puccinia graminis f. sp. tritici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XDS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xds OCA], [https://pdbe.org/7xds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xds RCSB], [https://www.ebi.ac.uk/pdbsum/7xds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xds ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[https://www.uniprot.org/uniprot/A0A5B0N367_PUCGR A0A5B0N367_PUCGR]] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. The direct recognition mechanism of pathogen effectors by coiled-coil NLRs (CNLs) remains unclear. We demonstrate that the Triticum monococcum CNL Sr35 directly recognizes the pathogen effector AvrSr35 from Puccinia graminis f. sp. tritici and report a cryo-electron microscopy structure of Sr35 resistosome and a crystal structure of AvrSr35. We show that AvrSr35 forms homodimers that are disassociated into monomers upon direct recognition by the leucine-rich repeat domain of Sr35, which induces Sr35 resistosome assembly and the subsequent immune response. The first 20 amino-terminal residues of Sr35 are indispensable for immune signaling but not for plasma membrane association. Our findings reveal the direct recognition and activation mechanism of a plant CNL and provide insights into biochemical function of Sr35 resistosome. | |||
Pathogen effector AvrSr35 triggers Sr35 resistosome assembly via a direct recognition mechanism.,Zhao YB, Liu MX, Chen TT, Ma X, Li ZK, Zheng Z, Zheng SR, Chen L, Li YZ, Tang LR, Chen Q, Wang P, Ouyang S Sci Adv. 2022 Sep 9;8(36):eabq5108. doi: 10.1126/sciadv.abq5108. Epub 2022 Sep 9. PMID:36083908<ref>PMID:36083908</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7xds" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Puccinia graminis f. sp. tritici]] | |||
[[Category: Ouyang SY]] | |||
[[Category: Zhao YB]] | |||
Revision as of 06:19, 28 September 2022
Crystal structure of wheat stem rust effector AvrSr35
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