1tc3

TRANSPOSASE TC3A1-65 FROM CAENORHABDITIS ELEGANS

spinqualitylabelsall models 1tc3, resolution 2.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1tc3 is a 3 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TC3A_CAEEL] Binds specifically to the terminal nucleotides of the TC3 inverted repeat. Its expression results in frequent excision and transposition of endogenous TC3 elements. TC3 transposase acts by making double strand breaks at the ends of TC3 element. The excised element would then be inserted into a target sequence. Publication Abstract from PubMed The crystal structure of the complex between the N-terminal DNA-binding domain of Tc3 transposase and an oligomer of transposon DNA has been determined. The specific DNA-binding domain contains three alpha-helices, of which two form a helix-turn-helix (HTH) motif. The recognition of transposon DNA by the transposase is mediated through base-specific contacts and complementarity between protein and sequence-dependent deformations of the DNA. The HTH motif makes four base-specific contacts with the major groove, and the N-terminus makes three base-specific contacts with the minor groove. The DNA oligomer adopts a non-linear B-DNA conformation, made possible by a stretch of seven G:C base pairs at one end and a TATA sequence towards the other end. Extensive contacts (seven salt bridges and 16 hydrogen bonds) of the protein with the DNA backbone allow the protein to probe and recognize the sequence-dependent DNA deformation. The DNA-binding domain forms a dimer in the crystals. Each monomer binds a separate transposon end, implying that the dimer plays a role in synapsis, necessary for the simultaneous cleavage of both transposon termini. Crystal structure of the specific DNA-binding domain of Tc3 transposase of C.elegans in complex with transposon DNA.,van Pouderoyen G, Ketting RF, Perrakis A, Plasterk RH, Sixma TK EMBO J. 1997 Oct 1;16(19):6044-54. PMID:9312061 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transposase 3D structures

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