BASIL2022GV3R8E: Difference between revisions

After various experiments and discussion, we have concluded that the novel protein 3R8E is a glucose kinase. By using an in-vitro assay, we obtained results comparing the phosphorylation rates of five sugars in the presence of our putative kinase. From the assay results. we were able to calculate an average specific activity for all experimental sugars, and by comparing these numbers we can clearly see that glucose is being phosphorylated in the presence of protein 3R8E. To get to this conclusion, we ran triplicates of the experiment for each sugar, and then followed a calculation procedure to get specific activity numbers to quantify how active our protein is with a given substrate. By doing this, we were able to validate and further support our hypothesis, which now can allow others to replicate or continue our research.

After validating our results, we now look to take our findings to a micropublication website for undergraduate research. By doing this, not only will our work be forward facing and available to the science community, but it also allows for collaboration and further questions to be asked. After completing the micropublication, we look to continue to develop research strategies for putative kinases, as the PDB has thousands of proteins with unsolved functions. We will do this by combining machine learning, data science, and lab work to allow undergraduate students and scientist to effectively research and study putative kinase structures and functions.