Sandbox Reserved 1750: Difference between revisions

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The SaCas9 recognizes the sgRNA scaffold within the <scene name='92/925538/Lobes_and_linkers/19'>REC lobe and WED domain</scene>. The WED contains five stranded beta sheets flanked with four alpha helices to allow binding of the repeat: anti-repeat duplex. REC lob binds the scaffold and secures it into the SaCas9 <ref name="Cas9" />.

== Endonuclease Activity of Cas9 ==

Finally, <scene name='92/925538/Lobes_and_linkers/12'>RuvC and HNH</scene> are in endonuclease activity (Scene). RuvC uses a two-metal ion mechanism of manganese to cleave the non-target DNA and causes a conformational change in L1. This conformational change leads to the phosphate group of the target strand to be cleaved by HNH. HNH includes a beta beta alpha metal fold and uses a one metal ion mechanism to cleave the target DNA <ref name="Cas9" />.

Finally, <scene name='92/925538/Lobes_and_linkers/12'>RuvC and HNH</scene> are in endonuclease activity (Scene). RuvC uses a two-metal ion mechanism of manganese to cleave the non-target DNA and causes a conformational change in L1. This is modeled as manganese however, it is often magnesium in cell. The magnesium allows a histidine to become a general base and cleave the target DNA. This conformational change leads to the phosphate group of the target strand to be cleaved by HNH. HNH includes a beta beta alpha metal fold and uses a one metal ion mechanism to cleave the target DNA <ref name="Cas9" />.

</structuresection>

==References==

Sandbox Reserved 1750: Difference between revisions

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@@ Line 10: / Line 10: @@
 The SaCas9 recognizes the sgRNA scaffold within the <scene name='92/925538/Lobes_and_linkers/19'>REC lobe and WED domain</scene>. The WED contains five stranded beta sheets flanked with four alpha helices to allow binding of the repeat: anti-repeat duplex. REC lob binds the scaffold and secures it into the SaCas9 <ref name="Cas9" />.
 == Endonuclease Activity of Cas9 ==
-Finally, <scene name='92/925538/Lobes_and_linkers/12'>RuvC and HNH</scene> are in endonuclease activity (Scene). RuvC uses a two-metal ion mechanism of manganese to cleave the non-target DNA and causes a conformational change in L1. This conformational change leads to the phosphate group of the target strand to be cleaved by HNH. HNH includes a beta beta alpha metal fold and uses a one metal ion mechanism to cleave the target DNA <ref name="Cas9" />.
+Finally, <scene name='92/925538/Lobes_and_linkers/12'>RuvC and HNH</scene> are in endonuclease activity (Scene). RuvC uses a two-metal ion mechanism of manganese to cleave the non-target DNA and causes a conformational change in L1. This is modeled as manganese however, it is often magnesium in cell. The magnesium allows a histidine to become a general base and cleave the target DNA. This conformational change leads to the phosphate group of the target strand to be cleaved by HNH. HNH includes a beta beta alpha metal fold and uses a one metal ion mechanism to cleave the target DNA <ref name="Cas9" />.
 </structuresection>
 ==References==
 <references />