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==Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea==
==Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea==
<StructureSection load='4flo' size='340' side='right' caption='[[4flo]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='4flo' size='340' side='right'caption='[[4flo]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4flo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"neisseria_polysacchareae"_riou_et_al._1983 "neisseria polysacchareae" riou et al. 1983]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FLO FirstGlance]. <br>
<table><tr><td colspan='2'>[[4flo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_polysaccharea Neisseria polysaccharea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FLO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g5a|1g5a]], [[4flq|4flq]], [[4flr|4flr]], [[4fls|4fls]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4flo OCA], [https://pdbe.org/4flo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4flo RCSB], [https://www.ebi.ac.uk/pdbsum/4flo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4flo ProSAT]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ams ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489 "Neisseria polysacchareae" Riou et al. 1983])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Amylosucrase Amylosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.4 2.4.1.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4flo OCA], [http://pdbe.org/4flo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4flo RCSB], [http://www.ebi.ac.uk/pdbsum/4flo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4flo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO]] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>
[https://www.uniprot.org/uniprot/AMYS_NEIPO AMYS_NEIPO] Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.<ref>PMID:9882648</ref>  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Neisseria polysacchareae riou et al. 1983]]
[[Category: Large Structures]]
[[Category: Amylosucrase]]
[[Category: Neisseria polysaccharea]]
[[Category: Andre, I]]
[[Category: Andre I]]
[[Category: Barbe, S]]
[[Category: Barbe S]]
[[Category: Champion, E]]
[[Category: Champion E]]
[[Category: Descroix, K]]
[[Category: Descroix K]]
[[Category: Guerin, F]]
[[Category: Guerin F]]
[[Category: Monsan, P]]
[[Category: Monsan P]]
[[Category: Morel, S]]
[[Category: Morel S]]
[[Category: Moulis, C]]
[[Category: Moulis C]]
[[Category: Mourey, L]]
[[Category: Mourey L]]
[[Category: Mulard, L A]]
[[Category: Mulard LA]]
[[Category: Remaud-Simeon, M]]
[[Category: Remaud-Simeon M]]
[[Category: Tran, T H]]
[[Category: Tran TH]]
[[Category: Tranier, S]]
[[Category: Tranier S]]
[[Category: Amylose synthesis]]
[[Category: Beta/alpha-barrel]]
[[Category: Carbohydrate]]
[[Category: Glucosyltransferase]]
[[Category: Glycoside hydrolase]]
[[Category: Sucrose isomerization]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/4flo"