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==X-ray Crystal Structure of a Ternary Complex of Double Bond Reductase from Nicotiana tabacum==
==X-ray Crystal Structure of a Ternary Complex of Double Bond Reductase from Nicotiana tabacum==
<StructureSection load='4hfn' size='340' side='right' caption='[[4hfn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='4hfn' size='340' side='right'caption='[[4hfn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4hfn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFN FirstGlance]. <br>
<table><tr><td colspan='2'>[[4hfn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HFN FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIY:(2E)-3-(4-HYDROXY-3-METHOXYPHENYL)PROP-2-ENAL'>CIY</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIY:(2E)-3-(4-HYDROXY-3-METHOXYPHENYL)PROP-2-ENAL'>CIY</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hfj|4hfj]], [[4hfm|4hfm]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfn OCA], [https://pdbe.org/4hfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hfn RCSB], [https://www.ebi.ac.uk/pdbsum/4hfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfn ProSAT]</span></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-alkenal_reductase_(NAD(P)(+)) 2-alkenal reductase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfn OCA], [http://pdbe.org/4hfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hfn RCSB], [http://www.ebi.ac.uk/pdbsum/4hfn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfn ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]  
[https://www.uniprot.org/uniprot/DBR_TOBAC DBR_TOBAC] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3]
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
*[[Alcohol dehydrogenase|Alcohol dehydrogenase]]
*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: American tobacco]]
[[Category: Large Structures]]
[[Category: Scrutton, N S]]
[[Category: Nicotiana tabacum]]
[[Category: Toogood, H S]]
[[Category: Scrutton NS]]
[[Category: Alkene reduction]]
[[Category: Toogood HS]]
[[Category: Oxidoreductase]]
[[Category: Rossmann fold]]
[[Category: Twisted b-barrel]]
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