7zhe: Difference between revisions
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==Crystal structure of CtaZ from Ruminiclostridium cellulolyticum== | |||
<StructureSection load='7zhe' size='340' side='right'caption='[[7zhe]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7zhe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminiclostridium_cellulolyticum Ruminiclostridium cellulolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZHE FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zhe OCA], [https://pdbe.org/7zhe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zhe RCSB], [https://www.ebi.ac.uk/pdbsum/7zhe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zhe ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B8I0Z6_RUMCH B8I0Z6_RUMCH] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Understanding antibiotic resistance mechanisms is central to the development of anti-infective therapies and genomics-based drug discovery. Yet, many knowledge gaps remain regarding the resistance strategies employed against novel types of antibiotics from less-explored producers such as anaerobic bacteria, among them the Clostridia. Through the use of genome editing and functional assays, we found that CtaZ confers self-resistance against the copper chelator and gyrase inhibitor closthioamide (CTA) in Ruminiclostridium cellulolyticum. Bioinformatics, biochemical analyses, and X-ray crystallography revealed CtaZ as a founding member of a new group of GyrI-like proteins. CtaZ is unique in binding a polythioamide scaffold in a ligand-optimized hydrophobic pocket, thereby confining CTA. By genome mining using CtaZ as a handle, we discovered previously overlooked homologs encoded by diverse members of the phylum Firmicutes, including many pathogens. In addition to characterizing both a new role for a GyrI-like domain in self-resistance and unprecedented thioamide binding, this work aids in uncovering related drug-resistance mechanisms. | |||
A Specialized Polythioamide-Binding Protein Confers Antibiotic Self-Resistance in Anaerobic Bacteria.,Gude F, Molloy EM, Horch T, Dell M, Dunbar KL, Krabbe J, Groll M, Hertweck C Angew Chem Int Ed Engl. 2022 Sep 12;61(37):e202206168. doi:, 10.1002/anie.202206168. Epub 2022 Aug 3. PMID:35852818<ref>PMID:35852818</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7zhe" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Horch | [[Category: Large Structures]] | ||
[[Category: | [[Category: Ruminiclostridium cellulolyticum]] | ||
[[Category: | [[Category: Dell M]] | ||
[[Category: Dunbar KL]] | |||
[[Category: Groll M]] | |||
[[Category: Gude F]] | |||
[[Category: Hertweck C]] | |||
[[Category: Horch T]] | |||
[[Category: Krabbe J]] | |||
[[Category: Molloy EM]] | |||
Revision as of 07:29, 9 November 2022
Crystal structure of CtaZ from Ruminiclostridium cellulolyticum
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