4iul: Difference between revisions

Revision as of 21:12, 16 November 2022

MIF4G domain of DAP5

Structural highlights

4iul is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IF4G2_HUMAN Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Death-associated protein 5 (DAP5/p97) is a homolog of the eukaryotic initiation factor 4G (eIF4G) that promotes the IRES-driven translation of multiple cellular mRNAs. Central to its function is the middle domain (MIF4G), which recruits the RNA helicase eIF4A. The middle domain of eIF4G consists of tandem HEAT repeats that coalesce to form a solenoid-type structure. Here, we report the crystal structure of the DAP5 MIF4G domain. Its overall fold is very similar to that of eIF4G; however, significant conformational variations impart distinct surface properties that could explain the observed differences in IRES binding between the two proteins. Interestingly, quantitative analysis of the DAP5-eIF4A interaction using isothermal titration calorimetry reveals a 10-fold lower affinity than with the eIF4G-eIF4A interaction that appears to affect their ability to stimulate eIF4A RNA unwinding activity in vitro. This difference in stability of the complex may have functional implications in selecting the mode of translation initiation.

Structural Analysis of the DAP5 MIF4G Domain and Its Interaction with eIF4A.,Virgili G, Frank F, Feoktistova K, Sawicki M, Sonenberg N, Fraser CS, Nagar B Structure. 2013 Mar 5. pii: S0969-2126(13)00023-3. doi:, 10.1016/j.str.2013.01.015. PMID:23478064^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Imataka H, Olsen HS, Sonenberg N. A new translational regulator with homology to eukaryotic translation initiation factor 4G. EMBO J. 1997 Feb 17;16(4):817-25. PMID:9049310 doi:10.1093/emboj/16.4.817
↑ Levy-Strumpf N, Deiss LP, Berissi H, Kimchi A. DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Mol Cell Biol. 1997 Mar;17(3):1615-25. PMID:9032289
↑ Pyronnet S, Dostie J, Sonenberg N. Suppression of cap-dependent translation in mitosis. Genes Dev. 2001 Aug 15;15(16):2083-93. PMID:11511540 doi:10.1101/gad.889201
↑ Henis-Korenblit S, Shani G, Sines T, Marash L, Shohat G, Kimchi A. The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins. Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5400-5. Epub 2002 Apr 9. PMID:11943866 doi:10.1073/pnas.082102499
↑ Virgili G, Frank F, Feoktistova K, Sawicki M, Sonenberg N, Fraser CS, Nagar B. Structural Analysis of the DAP5 MIF4G Domain and Its Interaction with eIF4A. Structure. 2013 Mar 5. pii: S0969-2126(13)00023-3. doi:, 10.1016/j.str.2013.01.015. PMID:23478064 doi:https://dx.doi.org/10.1016/j.str.2013.01.015

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Imataka H, Olsen HS, Sonenberg N. A new translational regulator with homology to eukaryotic translation initiation factor 4G. EMBO J. 1997 Feb 17;16(4):817-25. PMID:9049310 doi:10.1093/emboj/16.4.817

[2] Levy-Strumpf N, Deiss LP, Berissi H, Kimchi A. DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Mol Cell Biol. 1997 Mar;17(3):1615-25. PMID:9032289

[3] Pyronnet S, Dostie J, Sonenberg N. Suppression of cap-dependent translation in mitosis. Genes Dev. 2001 Aug 15;15(16):2083-93. PMID:11511540 doi:10.1101/gad.889201

[4] Henis-Korenblit S, Shani G, Sines T, Marash L, Shohat G, Kimchi A. The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins. Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5400-5. Epub 2002 Apr 9. PMID:11943866 doi:10.1073/pnas.082102499

[5] Virgili G, Frank F, Feoktistova K, Sawicki M, Sonenberg N, Fraser CS, Nagar B. Structural Analysis of the DAP5 MIF4G Domain and Its Interaction with eIF4A. Structure. 2013 Mar 5. pii: S0969-2126(13)00023-3. doi:, 10.1016/j.str.2013.01.015. PMID:23478064 doi:https://dx.doi.org/10.1016/j.str.2013.01.015

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[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
 ==MIF4G domain of DAP5==
-<StructureSection load='4iul' size='340' side='right' caption='[[4iul]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='4iul' size='340' side='right'caption='[[4iul]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4iul]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IUL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4iul]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IUL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DAP5, EIF4G2, OK/SW-cl.75 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iul OCA], [https://pdbe.org/4iul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iul RCSB], [https://www.ebi.ac.uk/pdbsum/4iul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iul ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iul OCA], [http://pdbe.org/4iul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iul RCSB], [http://www.ebi.ac.uk/pdbsum/4iul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iul ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IF4G2_HUMAN IF4G2_HUMAN]] Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.<ref>PMID:9049310</ref> <ref>PMID:9032289</ref> <ref>PMID:11511540</ref> <ref>PMID:11943866</ref>
+[https://www.uniprot.org/uniprot/IF4G2_HUMAN IF4G2_HUMAN] Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.<ref>PMID:9049310</ref> <ref>PMID:9032289</ref> <ref>PMID:11511540</ref> <ref>PMID:11943866</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 18: @@
 </div>
 <div class="pdbe-citations 4iul" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Feoktistova, K]]
+[[Category: Large Structures]]
-[[Category: Frank, F]]
+[[Category: Feoktistova K]]
-[[Category: Fraser, C]]
+[[Category: Frank F]]
-[[Category: Nagar, B]]
+[[Category: Fraser C]]
-[[Category: Sawicki, M]]
+[[Category: Nagar B]]
-[[Category: Sonenberg, N]]
+[[Category: Sawicki M]]
-[[Category: Virgili, G]]
+[[Category: Sonenberg N]]
-[[Category: Eif4a]]
+[[Category: Virgili G]]
-[[Category: Heat repeat]]
-[[Category: Protein-protein interaction]]
-[[Category: Translation]]

4iul: Difference between revisions

Revision as of 21:12, 16 November 2022

MIF4G domain of DAP5

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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