4j7a

Crystal Structure of Est25 - a Bacterial Homolog of Hormone-Sensitive Lipase from a Metagenomic Library

spinqualitylabelsall models 4j7a, resolution 1.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4j7a is a 4 chain structure with sequence from Uncultured bacterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q4TZQ3_9BACT Publication Abstract from PubMed Intracellular mobilization of fatty acids from triacylglycerols in mammalian adipose tissues proceeds through a series of lipolytic reactions. Among the enzymes involved, hormone-sensitive lipase (HSL) is noteworthy for its central role in energy homeostasis and the pathogenic role played by its dysregulation. By virtue of its broad substrate specificity, HSL may also serve as an industrial biocatalyst. In a previous report, Est25, a bacterial homologue of HSL, was identified from a metagenomic library by functional screening. Here, the crystal structure of Est25 is reported at 1.49 A resolution; it exhibits an alpha/beta-hydrolase fold consisting of a central beta-sheet enclosed by alpha-helices on both sides. The structural features of the cap domain, the substrate-binding pocket and the dimeric interface of Est25, together with biochemical and biophysical studies including native PAGE, mass spectrometry, dynamic light scattering, gel filtration and enzyme assays, could provide a basis for understanding the properties and regulation of hormone-sensitive lipase (HSL). The increased stability of cross-linked Est25 aggregates (CLEA-Est25) and their potential for extensive reuse support the application of this preparation as a biocatalyst in biotransformation processes. Structural and functional analyses of a bacterial homologue of hormone-sensitive lipase from a metagenomic library.,Ngo TD, Ryu BH, Ju H, Jang E, Park K, Kim KK, Kim TD Acta Crystallogr D Biol Crystallogr. 2013 Sep;69(Pt 9):1726-37. doi:, 10.1107/S0907444913013425. Epub 2013 Aug 15. PMID:23999296 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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