7xpl: Difference between revisions
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==Crystal structure of a C/D-free RNA-guided RNA 2'-O-methyltransferase== | |||
<StructureSection load='7xpl' size='340' side='right'caption='[[7xpl]], [[Resolution|resolution]] 2.21Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7xpl]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus] and [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_98/2 Saccharolobus solfataricus 98/2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XPL FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xpl OCA], [https://pdbe.org/7xpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xpl RCSB], [https://www.ebi.ac.uk/pdbsum/7xpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xpl ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0E3MJI1_SACSO A0A0E3MJI1_SACSO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Box C/D RNAs guide site-specific 2'-O-methylation of RNAs in archaea and eukaryotes. The defining feature of methylation guide RNAs is two sets of box C and D motifs that form kink-turn structures specifically recognized by L7Ae family proteins. Here, we engineered a new type of methylation guides that lack C/D motifs and require no L7Ae for assembly and function. We determined a crystal structure of a bipartite C/D-free guide RNA in complex with Nop5, fibrillarin and substrate in the active form at 2.2 A resolution. The stems of new guide RNAs functionally replace C/D motifs in Nop5 binding, precisely placing the substrate for site-specific modification. We also found that the bipartite architecture and association of L7Ae with C/D motifs enhance modification when association of guide RNAs or substrates is weak. Our study provides insights into the variations, robustness and possible evolutionary path of methylation guide RNAs. | |||
Methylation guide RNAs without box C/D motifs.,Wang J, Yang Z, Ye K RNA. 2022 Sep 20. pii: rna.079379.122. doi: 10.1261/rna.079379.122. PMID:36127125<ref>PMID:36127125</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7xpl" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharolobus solfataricus]] | |||
[[Category: Saccharolobus solfataricus 98/2]] | |||
[[Category: Wang J]] | |||
[[Category: Ye K]] | |||
Revision as of 08:33, 7 December 2022
Crystal structure of a C/D-free RNA-guided RNA 2'-O-methyltransferase
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