4kkp: Difference between revisions

Revision as of 08:56, 7 December 2022

Crystal structure of Vibrio cholerae RbmA (crystal form 2)

Structural highlights

4kkp is a 2 chain structure with sequence from Vibrio cholerae MJ-1236. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	MSE
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

During the transition from a free-swimming, single-cell life-style to a sessile, multicellular state called a biofilm, bacteria produce and secrete an extracellular matrix comprised of nucleic acids, exopolysaccharides and adhesion proteins. The Vibrio cholerae biofilm matrix contains three major protein components, RbmA, Bap1 and RbmC, which are unique to Vibrio cholerae and appear to support biofilm formation at particular steps in the process. Here, we focus on RbmA, a structural protein with an unknown fold. RbmA participates in the early cell-cell adhesion events, and is found throughout the biofilm where it localizes to cell-cell contact sites. We determined crystal structures of RbmA and revealed that the protein folds into tandem fibronectin III (FnIII) folds. The protein is dimeric in solution and in crystals, with the dimer interface displaying a surface groove that is lined with several positively charged residues. Structure-guided mutagenesis studies establish a crucial role for this surface patch for RbmA function. Based on the structure, we hypothesize that RbmA serves as a tether by maintaining flexible linkages between cells and the extracellular matrix.

Structural basis for biofilm formation via the Vibrio cholerae matrix protein RbmA.,Giglio KM, Fong JC, Yildiz FH, Sondermann H J Bacteriol. 2013 May 17. PMID:23687270^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Giglio KM, Fong JC, Yildiz FH, Sondermann H. Structural basis for biofilm formation via the Vibrio cholerae matrix protein RbmA. J Bacteriol. 2013 May 17. PMID:23687270 doi:10.1128/JB.00374-13

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OCA

[1] Giglio KM, Fong JC, Yildiz FH, Sondermann H. Structural basis for biofilm formation via the Vibrio cholerae matrix protein RbmA. J Bacteriol. 2013 May 17. PMID:23687270 doi:10.1128/JB.00374-13

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of Vibrio cholerae RbmA (crystal form 2)==
-<StructureSection load='4kkp' size='340' side='right' caption='[[4kkp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='4kkp' size='340' side='right'caption='[[4kkp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4kkp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibcj Vibcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KKP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4kkp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_MJ-1236 Vibrio cholerae MJ-1236]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KKP FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kkq|4kkq]], [[4kkr|4kkr]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kkp OCA], [https://pdbe.org/4kkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kkp RCSB], [https://www.ebi.ac.uk/pdbsum/4kkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kkp ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VCD_003406 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=593588 VIBCJ])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kkp OCA], [http://pdbe.org/4kkp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kkp RCSB], [http://www.ebi.ac.uk/pdbsum/4kkp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kkp ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 22: / Line 20: @@
 __TOC__
 </StructureSection>
-[[Category: Vibcj]]
+[[Category: Large Structures]]
-[[Category: Giglio, K M]]
+[[Category: Vibrio cholerae MJ-1236]]
-[[Category: Sondermann, H]]
+[[Category: Giglio KM]]
-[[Category: Fn-iii]]
+[[Category: Sondermann H]]
-[[Category: Matrix protein]]
-[[Category: Secreted]]
-[[Category: Structural protein]]

4kkp: Difference between revisions

Revision as of 08:56, 7 December 2022

Crystal structure of Vibrio cholerae RbmA (crystal form 2)

Structural highlights

Publication Abstract from PubMed

References

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