Sandbox Reserved 1759: Difference between revisions
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== Biological relevance and broader implications == | == Biological relevance and broader implications == | ||
IP | |||
Understanding the components of the mevalonate pathway | |||
== Important amino acids== | == Important amino acids== | ||
The <scene name='93/934003/Ligand/ | The <scene name='93/934003/Ligand/2'>ligand of interest</scene> in the MBD enzyme is called <scene name='93/934003/Ola/1'>Oleic acid (OLA)</scene> and is located within subunit A. OLA is hydrogen bonded to <scene name='93/934003/Arg128/2'>Arg128</scene>. In addition to the hydrogen bonding between OLA and Arg128, OLA is also hydrogen bonded to a water molecule which is hydrogen bonded to Arg128 as well. Amino acids residue aspartate is important for catalytic activity and the stability of the protein. When <scene name='93/934003/Asp309/2'>Asp309</scene> is replaced the enzyme experiences complete loss of MBD activity. The internal surface of the ligand binding cavity consists of mostly non-polar amino acids. The cavity opening is mostly polar amino acids, such as <scene name='93/934003/Cavity_polar_residue/1'>Lys94, Tyr99, Arg128, and Glu138</scene>. | ||
== Structural highlights == | == Structural highlights == | ||
