Sandbox Reserved 1761: Difference between revisions
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== Important amino acids== | == Important amino acids== | ||
The ligand of ''h''OAT is Pyridoxal-5'-Phosphate <scene name='93/934005/Plp/1'>(PLP)</scene>. The role of the catalytic amino acids in an enzyme is to bind to a substrate, changing the structure, causing bonds to break and new bonds to be formed. When there is a difficult reaction, the triad of amino acids works in tandem to facilitate the reaction. A <scene name='93/934005/Glu235/1'>Glu 235</scene>-<scene name='93/934005/Arg413/1'>Arg 413</scene> <scene name='93/934005/Arg_413_glu_235/1'>salt bridge</scene> was found on AVA, but not on GABA. In regards to GABA, the salt bridge was disrupted in 30% of the monomers. | The ligand of ''h''OAT is Pyridoxal-5'-Phosphate <scene name='93/934005/Plp/1'>(PLP)</scene>. The role of the catalytic amino acids in an enzyme is to bind to a substrate, changing the structure, causing bonds to break and new bonds to be formed. When there is a difficult reaction, the triad of amino acids works in tandem to facilitate the reaction. A <scene name='93/934005/Glu235/1'>Glu 235</scene>-<scene name='93/934005/Arg413/1'>Arg 413</scene> <scene name='93/934005/Arg_413_glu_235/1'>salt bridge</scene> was found on AVA, but not on GABA. In regards to GABA, the salt bridge was disrupted in 30% of the monomers. | ||
The crystal soaking experiments created an opportunity to decipher the reaction intermediates of the structures. While GABA linked to PLP covalently, PLP and catalytic Lys 292 were linked by AVA. | The crystal soaking experiments created an opportunity to decipher the reaction intermediates of the structures. While GABA linked to PLP covalently, PLP and catalytic <scene name='93/934005/Lys_292/1'>Lys 292</scene> were linked by AVA. | ||
== Structural highlights == | == Structural highlights == | ||
