4m1r

Structure of a novel cellulase 5 from a sugarcane soil metagenomic library

spinqualitylabelsall models 4m1r, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4m1r is a 2 chain structure with sequence from Soil metagenome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: TRS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Cellulases play a key role in enzymatic routes for degradation of plant cell-wall polysaccharides into simple and economically-relevant sugars. However, their low performance on complex substrates and reduced stability under industrial conditions remain the main obstacle for the large-scale production of cellulose-derived products and biofuels. Thus, in this study a novel cellulase with unusual catalytic properties from sugarcane soil metagenome (CelE1) was isolated and characterized. The polypeptide deduced from the celE1 gene encodes a unique glycoside hydrolase domain belonging to GH5 family. The recombinant enzyme was active on both carboxymethyl cellulose and beta-glucan with an endo-acting mode according to capillary electrophoretic analysis of cleavage products. CelE1 showed optimum hydrolytic activity at pH 7.0 and 50 degrees C with remarkable activity at alkaline conditions that is attractive for industrial applications in which conventional acidic cellulases are not suitable. Moreover, its three-dimensional structure was determined at 1.8 A resolution that allowed the identification of an insertion of eight residues in the beta8-alpha8 loop of the catalytic domain of CelE1, which is not conserved in its psychrophilic orthologs. This 8-residue-long segment is a prominent and distinguishing feature of thermotolerant cellulases 5 suggesting that it might be involved with thermal stability. Based on its unconventional characteristics, CelE1 could be potentially employed in biotechnological processes that require thermotolerant and alkaline cellulases. Structure and function of a novel cellulase 5 from sugarcane soil metagenome.,Alvarez TM, Paiva JH, Ruiz DM, Cairo JP, Pereira IO, Paixao DA, de Almeida RF, Tonoli CC, Ruller R, Santos CR, Squina FM, Murakami MT PLoS One. 2013 Dec 17;8(12):e83635. doi: 10.1371/journal.pone.0083635., eCollection 2013. PMID:24358302 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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