4mhr

Crystal structure of EctD from S. alaskensis in its apoform

spinqualitylabelsall models 4mhr, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4mhr is a 1 chain structure with sequence from Sphingopyxis alaskensis RB2256. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ECTD_SPHAL Involved in the biosynthesis of 5-hydroxyectoine, called compatible solute, which helps organisms to survive extreme osmotic stress by acting as a highly soluble organic osmolyte. Catalyzes the 2-oxoglutarate-dependent selective hydroxylation of L-ectoine to yield (4S,5S)-5-hydroxyectoine. Publication Abstract from PubMed Ectoine and its derivative 5-hydroxyectoine are compatible solutes that are widely synthesized by Bacteria to cope physiologically with osmotic stress. They also serve as chemical chaperones and maintain the functionality of macromolecules. 5-hydroxyectoine is produced from ectoine through a stereo-specific hydroxylation, an enzymatic reaction catalyzed by the ectoine hydroxylase (EctD). The EctD protein is a member of the non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase superfamily and is evolutionarily well conserved. We studied the ectoine hydroxylase from the cold-adapted marine ultra-microbacterium Sphingopyxis alaskensis (Sa) and found that the purified SaEctD protein is a homo-dimer in solution. We determined the SaEctD crystal structure in its apo-form, complexed with the iron catalyst and in a form that contained iron, the co-substrate 2-oxoglutarate, and the reaction product of EctD, 5-hydroxyectoine. The iron and 2-oxoglutarate ligands are bound within the EctD active site in a fashion similar to that found in other members of the dioxygenase superfamily. 5-hydroxyectoine on the other hand, is coordinated by EctD in manner different from that found in high-affinity solute receptor proteins operating in conjunction with microbial import systems for ectoines. Our crystallographic analysis provides a detailed view into the active site of the ectoine hydroxylase and exposes an intricate network of interactions between the enzyme and its ligands that collectively ensure the hydroxylation of the ectoine substrate in a position- and stereo-specific manner. Crystal Structure of the Ectoine Hydroxylase: a Snapshot of the Active Site.,Hoeppner A, Widderich N, Lenders M, Bremer E, Smits SH J Biol Chem. 2014 Aug 29. pii: jbc.M114.576769. PMID:25172507 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Hydroxylases 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA