8bda: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 8bda is ~~ON HOLD until Paper~~ Publication

==IFTA complex in anterograde Intraflagellar transport trains (Chlamydomonas reinhardtii)==

== Structural highlights ==

<table><tr><td colspan='2'>[[8bda]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BDA FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bda OCA], [https://pdbe.org/8bda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bda RCSB], [https://www.ebi.ac.uk/pdbsum/8bda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bda ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/A8JFR3_CHLRE A8JFR3_CHLRE]

== Publication Abstract from PubMed ==

Anterograde intraflagellar transport (IFT) trains are essential for cilia assembly and maintenance. These trains are formed of 22 IFT-A and IFT-B proteins that link structural and signaling cargos to microtubule motors for import into cilia. It remains unknown how the IFT-A/-B proteins are arranged into complexes and how these complexes polymerize into functional trains. Here we use in situ cryo-electron tomography of Chlamydomonas reinhardtii cilia and AlphaFold2 protein structure predictions to generate a molecular model of the entire anterograde train. We show how the conformations of both IFT-A and IFT-B are dependent on lateral interactions with neighboring repeats, suggesting that polymerization is required to cooperatively stabilize the complexes. Following three-dimensional classification, we reveal how IFT-B extends two flexible tethers to maintain a connection with IFT-A that can withstand the mechanical stresses present in actively beating cilia. Overall, our findings provide a framework for understanding the fundamental processes that govern cilia assembly.

~~Authors~~:

The molecular structure of IFT-A and IFT-B in anterograde intraflagellar transport trains.,Lacey SE, Foster HE, Pigino G Nat Struct Mol Biol. 2023 Jan 2. doi: 10.1038/s41594-022-00905-5. PMID:36593313<ref>PMID:36593313</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Chlamydomonas reinhardtii]]

[[Category: Large Structures]]

[[Category: Foster HE]]

[[Category: Lacey SE]]

[[Category: Pigino G]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8bda is ON HOLD  until Paper Publication
+==IFTA complex in anterograde Intraflagellar transport trains (Chlamydomonas reinhardtii)==
+<StructureSection load='8bda' size='340' side='right'caption='[[8bda]], [[Resolution|resolution]] 20.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8bda]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BDA FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bda OCA], [https://pdbe.org/8bda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bda RCSB], [https://www.ebi.ac.uk/pdbsum/8bda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bda ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A8JFR3_CHLRE A8JFR3_CHLRE]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Anterograde intraflagellar transport (IFT) trains are essential for cilia assembly and maintenance. These trains are formed of 22 IFT-A and IFT-B proteins that link structural and signaling cargos to microtubule motors for import into cilia. It remains unknown how the IFT-A/-B proteins are arranged into complexes and how these complexes polymerize into functional trains. Here we use in situ cryo-electron tomography of Chlamydomonas reinhardtii cilia and AlphaFold2 protein structure predictions to generate a molecular model of the entire anterograde train. We show how the conformations of both IFT-A and IFT-B are dependent on lateral interactions with neighboring repeats, suggesting that polymerization is required to cooperatively stabilize the complexes. Following three-dimensional classification, we reveal how IFT-B extends two flexible tethers to maintain a connection with IFT-A that can withstand the mechanical stresses present in actively beating cilia. Overall, our findings provide a framework for understanding the fundamental processes that govern cilia assembly.
-Authors:
+The molecular structure of IFT-A and IFT-B in anterograde intraflagellar transport trains.,Lacey SE, Foster HE, Pigino G Nat Struct Mol Biol. 2023 Jan 2. doi: 10.1038/s41594-022-00905-5. PMID:36593313<ref>PMID:36593313</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8bda" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Chlamydomonas reinhardtii]]
+[[Category: Large Structures]]
+[[Category: Foster HE]]
+[[Category: Lacey SE]]
+[[Category: Pigino G]]