4nkb: Difference between revisions

Revision as of 08:32, 11 January 2023

Crystal Structure of the cryptic polo box (CPB)of ZYG-1

Structural highlights

4nkb is a 2 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	DTT, MG, TRS
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ZYG1_CAEEL Protein kinase that plays a central role in centrosome duplication. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Plk4 family kinases control centriole assembly. Plk4s target mother centrioles through an interaction between their cryptic polo box (CPB) and acidic regions in the centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152. Here, we report a crystal structure for the CPB of C. elegans ZYG-1, which forms a Z-shaped dimer containing an intermolecular beta sheet with an extended basic surface patch. Biochemical and in vivo analysis revealed that electrostatic interactions dock the ZYG-1 CPB basic patch onto the SPD-2-derived acidic region to promote ZYG-1 targeting and new centriole assembly. Analysis of a different crystal form of the Drosophila Plk4 (DmPlk4) CPB suggests that it also forms a Z-shaped dimer. Comparison of the ZYG-1 and DmPlk4 CPBs revealed structural changes in the ZYG-1 CPB that confer selectivity for binding SPD-2 over Asterless-derived acidic regions. Overall, our findings suggest a conserved mechanism for centriolar docking of Plk4 homologs that initiate daughter centriole assembly.

Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases.,Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G Structure. 2014 Aug 5;22(8):1090-104. doi: 10.1016/j.str.2014.05.009. Epub 2014, Jun 26. PMID:24980795^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ O'Connell KF, Caron C, Kopish KR, Hurd DD, Kemphues KJ, Li Y, White JG. The C. elegans zyg-1 gene encodes a regulator of centrosome duplication with distinct maternal and paternal roles in the embryo. Cell. 2001 May 18;105(4):547-58. PMID:11371350
↑ Delattre M, Leidel S, Wani K, Baumer K, Bamat J, Schnabel H, Feichtinger R, Schnabel R, Gonczy P. Centriolar SAS-5 is required for centrosome duplication in C. elegans. Nat Cell Biol. 2004 Jul;6(7):656-64. PMID:15232593 doi:https://dx.doi.org/10.1038/ncb1146
↑ Leidel S, Delattre M, Cerutti L, Baumer K, Gonczy P. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat Cell Biol. 2005 Feb;7(2):115-25. PMID:15665853 doi:ncb1220
↑ Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G. Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases. Structure. 2014 Aug 5;22(8):1090-104. doi: 10.1016/j.str.2014.05.009. Epub 2014, Jun 26. PMID:24980795 doi:https://dx.doi.org/10.1016/j.str.2014.05.009

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] O'Connell KF, Caron C, Kopish KR, Hurd DD, Kemphues KJ, Li Y, White JG. The C. elegans zyg-1 gene encodes a regulator of centrosome duplication with distinct maternal and paternal roles in the embryo. Cell. 2001 May 18;105(4):547-58. PMID:11371350

[2] Delattre M, Leidel S, Wani K, Baumer K, Bamat J, Schnabel H, Feichtinger R, Schnabel R, Gonczy P. Centriolar SAS-5 is required for centrosome duplication in C. elegans. Nat Cell Biol. 2004 Jul;6(7):656-64. PMID:15232593 doi:https://dx.doi.org/10.1038/ncb1146

[3] Leidel S, Delattre M, Cerutti L, Baumer K, Gonczy P. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat Cell Biol. 2005 Feb;7(2):115-25. PMID:15665853 doi:ncb1220

[4] Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G. Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases. Structure. 2014 Aug 5;22(8):1090-104. doi: 10.1016/j.str.2014.05.009. Epub 2014, Jun 26. PMID:24980795 doi:https://dx.doi.org/10.1016/j.str.2014.05.009

[1]

[2]

[3]

[4]

@@ Line 3: / Line 3: @@
 <StructureSection load='4nkb' size='340' side='right'caption='[[4nkb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4nkb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NKB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NKB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4nkb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NKB FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nk7|4nk7]], [[4g7n|4g7n]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nkb OCA], [https://pdbe.org/4nkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nkb RCSB], [https://www.ebi.ac.uk/pdbsum/4nkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nkb ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C. elegans ZYG-1 (residues 338-564), F59E12.2, zyg-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nkb OCA], [http://pdbe.org/4nkb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nkb RCSB], [http://www.ebi.ac.uk/pdbsum/4nkb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nkb ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ZYG1_CAEEL ZYG1_CAEEL]] Protein kinase that plays a central role in centrosome duplication. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome.<ref>PMID:11371350</ref> <ref>PMID:15232593</ref> <ref>PMID:15665853</ref>
+[https://www.uniprot.org/uniprot/ZYG1_CAEEL ZYG1_CAEEL] Protein kinase that plays a central role in centrosome duplication. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome.<ref>PMID:11371350</ref> <ref>PMID:15232593</ref> <ref>PMID:15665853</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Non-specific serine/threonine protein kinase]]
+[[Category: Dong G]]
-[[Category: Dong, G]]
+[[Category: Shimanovskaya E]]
-[[Category: Shimanovskaya, E]]
-[[Category: Centriole biogenesis]]
-[[Category: Centrosome]]
-[[Category: Cryptic polo box]]
-[[Category: Transferase]]

4nkb: Difference between revisions

Revision as of 08:32, 11 January 2023

Crystal Structure of the cryptic polo box (CPB)of ZYG-1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools