4nsr

Crystal structure of the Immunity protein

spinqualitylabelsall models 4nsr, resolution 2.79Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4nsr is a 6 chain structure with sequence from Vibrio cholerae O1 biovar El Tor str. N16961. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TSIV3_VIBCH Immunity protein that plays a role in preventing early activation of toxin VgrG3. Publication Abstract from PubMed The bacterial type VI secretion system (T6SS) is used by donor cells to inject toxic effectors into receptor cells. The donor cells produce the corresponding immunity proteins to protect themselves against the effector proteins, thereby preventing their self-intoxication. Recently, the C-terminal domain of VgrG3 was identified as a T6SS effector. Information on the molecular mechanism of VgrG3 and its immunity protein TsaB has been lacking. Here, we determined the crystal structures of native TsaB and the VgrG3C-TsaB complex. VgrG3C adopts a canonical phage-T4-lysozyme-like fold. TsaB interacts with VgrG3C through molecular mimicry, and inserts into the VgrG3C pocket. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: VgrG3 and TsaBbind by x-ray crystallography (View interaction) TsaB and TsaBbind by x-ray crystallography (View interaction) VgrG3 and TsaBbind by cosedimentation in solution (View interaction) TsaB and TsaBbind by cosedimentation in solution (1,2) TsaBbinds to VgrG3 by surface plasmon resonance (1, 2, 3, 4, 5, 6, 7) VgrG3 and TsaBbind by molecular sieving (View interaction) TsaB and TsaBbind by molecular sieving (View interaction) VgrG3 and TsaBbind by x ray scattering (View interaction). Structural basis for recognition of the type VI spike protein VgrG3 by a cognate immunity protein.,Zhang J, Zhang H, Gao Z, Hu H, Dong C, Dong YH FEBS Lett. 2014 May 21;588(10):1891-8. doi: 10.1016/j.febslet.2014.04.016. Epub, 2014 Apr 18. PMID:24751834 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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