4o6z

Crystal structure of serine hydroxymethyltransferase with covalently bound PLP Schiff-base from Plasmodium falciparum

spinqualitylabelsall models 4o6z, resolution 2.98Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4o6z is a 4 chain structure with sequence from Plasmodium falciparum 3D7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: PLP, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GLYA_PLAF7 Catalyzes the interconversion of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, a source of one-carbon units used by thymidylate synthase to convert dUMP to dTMP for DNA synthesis (PubMed:11071283, PubMed:19591883, PubMed:24914963). Binds to its own mRNA and to the mRNA of bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) in vitro; the physiological relevance of this interaction is not clear (PubMed:19591883). Publication Abstract from PubMed Plasmodium falciparum serine hydroxymethyltransferase (PfSHMT), an enzyme in the dTMP synthesis cycle, is an antimalarial target because inhibition of its expression or function has been shown to be lethal to the parasite. As the wild-type enzyme could not be crystallized, protein engineering of residues on the surface was carried out. The surface-engineered mutant PfSHMT-F292E was successfully crystallized and its structure was determined at 3 A resolution. The PfSHMT-F292E structure is a good representation of PfSHMT as this variant revealed biochemical properties similar to those of the wild type. Although the overall structure of PfSHMT is similar to those of other SHMTs, unique features including the presence of two loops and a distinctive cysteine pair formed by Cys125 and Cys364 in the tetrahydrofolate (THF) substrate binding pocket were identified. These structural characteristics have never been reported in other SHMTs. Biochemical characterization and mutation analysis of these two residues confirm that they act as a disulfide/sulfhydryl switch to regulate the THF-dependent catalytic function of the enzyme. This redox switch is not present in the human enzyme, in which the cysteine pair is absent. The data reported here can be further exploited as a new strategy to specifically disrupt the activity of the parasite enzyme without interfering with the function of the human enzyme. The structure of Plasmodium falciparum serine hydroxymethyltransferase reveals a novel redox switch that regulates its activities.,Chitnumsub P, Ittarat W, Jaruwat A, Noytanom K, Amornwatcharapong W, Pornthanakasem W, Chaiyen P, Yuthavong Y, Leartsakulpanich U Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1517-27. doi:, 10.1107/S1399004714005598. Epub 2014 May 23. PMID:24914963 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Serine hydroxymethyltransferase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA