4oku: Difference between revisions

Revision as of 07:30, 25 January 2023

Structure of Toxoplasma gondii proMIC2

Structural highlights

4oku is a 2 chain structure with sequence from Toxoplasma gondii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	MAN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O00816_TOXGO

Publication Abstract from PubMed

Micronemal protein 2 (MIC2) is the key adhesin that supports gliding motility and host cell invasion by Toxoplasma gondii. With a von Willebrand factor A (VWA) domain and six thrombospondin repeat domains (TSR1-6) in its ectodomain, MIC2 connects to the parasite actomyosin system through its cytoplasmic tail. MIC2-associated protein (M2AP) binds noncovalently to the MIC2 ectodomain. MIC2 and M2AP are stored in micronemes as proforms. We find that the MIC2-M2AP ectodomain complex is a highly elongated 1:1 monomer with M2AP bound to the TSR6 domain. Crystal structures of N-terminal fragments containing the VWA and TSR1 domains for proMIC2 and MIC2 reveal a closed conformation of the VWA domain and how it associates with the TSR1 domain. A long, proline-rich, disulfide-bonded pigtail loop in TSR1 overlaps the VWA domain. Mannose alpha-C-linked to Trp-276 in TSR1 has an unusual (1)C4 chair conformation. The MIC2 VWA domain includes a mobile alpha5-helix and a 22-residue disordered region containing two disulfide bonds in place of an alpha6-helix. A hydrophobic residue in the prodomain binds to a pocket adjacent to the alpha7-helix that pistons in opening of the VWA domain to a putative high-affinity state.

Structures of the Toxoplasma gliding motility adhesin.,Song G, Springer TA Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4862-7. doi:, 10.1073/pnas.1403059111. Epub 2014 Mar 17. PMID:24639528^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Song G, Springer TA. Structures of the Toxoplasma gliding motility adhesin. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4862-7. doi:, 10.1073/pnas.1403059111. Epub 2014 Mar 17. PMID:24639528 doi:https://dx.doi.org/10.1073/pnas.1403059111

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Song G, Springer TA. Structures of the Toxoplasma gliding motility adhesin. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4862-7. doi:, 10.1073/pnas.1403059111. Epub 2014 Mar 17. PMID:24639528 doi:https://dx.doi.org/10.1073/pnas.1403059111

[1]

@@ Line 1: / Line 1: @@
 ==Structure of Toxoplasma gondii proMIC2==
-<StructureSection load='4oku' size='340' side='right' caption='[[4oku]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+<StructureSection load='4oku' size='340' side='right'caption='[[4oku]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4oku]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OKU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4oku]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OKU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4okr|4okr]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oku OCA], [https://pdbe.org/4oku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oku RCSB], [https://www.ebi.ac.uk/pdbsum/4oku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oku ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TGVEG_201780 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oku OCA], [http://pdbe.org/4oku PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oku RCSB], [http://www.ebi.ac.uk/pdbsum/4oku PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oku ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O00816_TOXGO O00816_TOXGO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Toxgo]]
+[[Category: Large Structures]]
-[[Category: Song, G]]
+[[Category: Toxoplasma gondii]]
-[[Category: Springer, T A]]
+[[Category: Song G]]
-[[Category: Adhesin]]
+[[Category: Springer TA]]
-[[Category: Cell adhesion]]
-[[Category: Gliding motility]]
-[[Category: Integrin i domain]]
-[[Category: Tsr domain]]
-[[Category: Vwa]]

4oku: Difference between revisions

Revision as of 07:30, 25 January 2023

Structure of Toxoplasma gondii proMIC2

Structural highlights

Function

Publication Abstract from PubMed

References

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