7xp7: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
The | ==Crystal Structure of the Flavoprotein ColB1 Catalyzing Assembly Line-Tethered Cysteine Dehydrogenation== | ||
<StructureSection load='7xp7' size='340' side='right'caption='[[7xp7]], [[Resolution|resolution]] 1.95Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7xp7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_filamentosus_NRRL_11379 Streptomyces filamentosus NRRL 11379]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XP7 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xp7 OCA], [https://pdbe.org/7xp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xp7 RCSB], [https://www.ebi.ac.uk/pdbsum/7xp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xp7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/W9FXI8_STRFL W9FXI8_STRFL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The recruitment of trans-acting enzymes by nonribosomal peptide synthetase (NRPS) assembly line is rarely reported. ColB1 is a flavin-dependent dehydrogenase that is recruited by an NRPS terminal condensation domain (Ct domain) and catalyzes peptidyl carrier protein (PCP)-tethered cysteine dehydrogenation in collismycin biosynthesis. We here report the crystal structure of ColB1 complexed with FAD and reveal a typical structural fold of acyl-CoA dehydrogenases (ACADs). However, ColB1 shows distinct structural features from ACADs in substrate recognition both at the entrance of and inside the active site. Site-directed mutagenesis and substrate modeling establish a Glu393-mediated catalytic mechanism, by which the cysteine substrate is sandwiched between Glu393 and FAD to facilitate C(alpha) proton abstraction and C(beta) hydride migration. A ColB1-PCP-Ct complex model is generated, providing structural basis for the unique recruitment interactions between ColB1 and the associated NRPS. These results add insights into the mechanisms by which trans-acting enzymes function in an assembly line. | |||
Structure-Based Mechanistic Insights into ColB1, a Flavoprotein Functioning in-trans in the 2,2'-Bipyridine Assembly Line for Cysteine Dehydrogenation.,Ma X, Tang Z, Ding W, Liu T, Yang D, Liu W, Ma M ACS Chem Biol. 2023 Jan 20;18(1):18-24. doi: 10.1021/acschembio.2c00785. Epub , 2023 Jan 5. PMID:36603145<ref>PMID:36603145</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 7xp7" style="background-color:#fffaf0;"></div> | ||
[[Category: Liu | == References == | ||
[[Category: | <references/> | ||
[[Category: Ma | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces filamentosus NRRL 11379]] | |||
[[Category: Liu W]] | |||
[[Category: Ma M]] | |||
[[Category: Ma XY]] | |||
[[Category: Tang ZJ]] | |||
Revision as of 06:43, 8 February 2023
Crystal Structure of the Flavoprotein ColB1 Catalyzing Assembly Line-Tethered Cysteine Dehydrogenation
| ||||||||||||
