4q80: Difference between revisions

Revision as of 07:47, 8 February 2023

Neutrophil serine protease 4 (PRSS57) with val-leu-lys-chloromethylketone (VLK-cmk)

Structural highlights

4q80 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	2YS, NAG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRS57_HUMAN

Publication Abstract from PubMed

Trypsin-fold proteases, the largest mammalian protease family, are classified by their primary substrate specificity into one of three categories, trypsin-like, chymotrypsin-like, and elastase-like, based on key structural features of their active site. However, the recently discovered neutrophil serine protease 4 (NSP4, also known as PRSS57) presents a paradox: NSP4 exhibits a trypsin-like specificity for cleaving substrates after arginine residues, but it bears elastase-like specificity determining residues in the active site. Here we show that NSP4 has a fully occluded S1 pocket and that the substrate P1-arginine adopts a noncanonical "up" conformation stabilized by a solvent-exposed H-bond network. This uncommon arrangement, conserved in all NSP4 orthologs, enables NSP4 to process substrates after both arginine as well as post-translationally modified arginine residues, such as methylarginine and citrulline. These findings establish a distinct paradigm for substrate recognition by a trypsin-fold protease and provide insights into the function of NSP4.

Structures of Neutrophil Serine Protease 4 Reveal an Unusual Mechanism of Substrate Recognition by a Trypsin-Fold Protease.,Lin SJ, Dong KC, Eigenbrot C, van Lookeren Campagne M, Kirchhofer D Structure. 2014 Aug 19. pii: S0969-2126(14)00238-X. doi:, 10.1016/j.str.2014.07.008. PMID:25156428^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lin SJ, Dong KC, Eigenbrot C, van Lookeren Campagne M, Kirchhofer D. Structures of Neutrophil Serine Protease 4 Reveal an Unusual Mechanism of Substrate Recognition by a Trypsin-Fold Protease. Structure. 2014 Aug 19. pii: S0969-2126(14)00238-X. doi:, 10.1016/j.str.2014.07.008. PMID:25156428 doi:https://dx.doi.org/10.1016/j.str.2014.07.008

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OCA

[1] Lin SJ, Dong KC, Eigenbrot C, van Lookeren Campagne M, Kirchhofer D. Structures of Neutrophil Serine Protease 4 Reveal an Unusual Mechanism of Substrate Recognition by a Trypsin-Fold Protease. Structure. 2014 Aug 19. pii: S0969-2126(14)00238-X. doi:, 10.1016/j.str.2014.07.008. PMID:25156428 doi:https://dx.doi.org/10.1016/j.str.2014.07.008

[1]

@@ Line 1: / Line 1: @@
 ==Neutrophil serine protease 4 (PRSS57) with val-leu-lys-chloromethylketone (VLK-cmk)==
-<StructureSection load='4q80' size='340' side='right' caption='[[4q80]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
+<StructureSection load='4q80' size='340' side='right'caption='[[4q80]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4q80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q80 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4q80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q80 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2YS:D-VALYL-N-[(2S,3S)-7-AMINO-1-CHLORO-2-HYDROXYHEPTAN-3-YL]-L-LEUCINAMIDE'>2YS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2YS:D-VALYL-N-[(2S,3S)-7-AMINO-1-CHLORO-2-HYDROXYHEPTAN-3-YL]-L-LEUCINAMIDE'>2YS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4q7x|4q7x]], [[4q7y|4q7y]], [[4q7z|4q7z]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q80 OCA], [https://pdbe.org/4q80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q80 RCSB], [https://www.ebi.ac.uk/pdbsum/4q80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q80 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRSS57, PRSSL1, UNQ782/PRO1599 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q80 OCA], [http://pdbe.org/4q80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q80 RCSB], [http://www.ebi.ac.uk/pdbsum/4q80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q80 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/PRS57_HUMAN PRS57_HUMAN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Dong, K C]]
+[[Category: Large Structures]]
-[[Category: Eigenbrot, C]]
+[[Category: Dong KC]]
-[[Category: Lin, S J]]
+[[Category: Eigenbrot C]]
-[[Category: Hydrolase-hydrolase inhibitor complex]]
+[[Category: Lin SJ]]
-[[Category: Peptidase]]
-[[Category: Trypsin homology]]

4q80: Difference between revisions

Revision as of 07:47, 8 February 2023

Neutrophil serine protease 4 (PRSS57) with val-leu-lys-chloromethylketone (VLK-cmk)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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