4ql0: Difference between revisions

Revision as of 11:34, 15 February 2023

Crystal Structure Analysis of the Membrane Transporter FhaC (double mutant V169T, I176N)

Structural highlights

4ql0 is a 1 chain structure with sequence from Bordetella pertussis Tohama I. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	1PE, BOG, P6G, PEG, PG4, PGE, PO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FHAC_BORPE Member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin (FHA).^[1]

Publication Abstract from PubMed

Omp85 proteins mediate translocation of polypeptide substrates across and into cellular membranes. They share a common architecture comprising substrate-interacting POTRA domains, a C-terminal 16-stranded beta-barrel pore and two signature motifs located on the inner barrel wall and at the tip of the extended L6 loop. The observation of two distinct conformations of the L6 loop in the available Omp85 structures previously suggested a functional role of conformational changes in L6 in the Omp85 mechanism. Here we present a 2.5 A resolution structure of a variant of the Omp85 secretion protein FhaC, in which the two signature motifs interact tightly and form the conserved 'lid lock'. Reanalysis of previous structural data shows that L6 adopts the same, conserved resting state position in all available Omp85 structures. The FhaC variant structure further reveals a competitive mechanism for the regulation of substrate binding mediated by the linker to the N-terminal plug helix H1.

Conserved Omp85 lid-lock structure and substrate recognition in FhaC.,Maier T, Clantin B, Gruss F, Dewitte F, Delattre AS, Jacob-Dubuisson F, Hiller S, Villeret V Nat Commun. 2015 Jun 10;6:7452. doi: 10.1038/ncomms8452. PMID:26058369^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hodak H, Clantin B, Willery E, Villeret V, Locht C, Jacob-Dubuisson F. Secretion signal of the filamentous haemagglutinin, a model two-partner secretion substrate. Mol Microbiol. 2006 Jul;61(2):368-82. Epub 2006 Jun 12. PMID:16771844 doi:10.1111/j.1365-2958.2006.05242.x
↑ Maier T, Clantin B, Gruss F, Dewitte F, Delattre AS, Jacob-Dubuisson F, Hiller S, Villeret V. Conserved Omp85 lid-lock structure and substrate recognition in FhaC. Nat Commun. 2015 Jun 10;6:7452. doi: 10.1038/ncomms8452. PMID:26058369 doi:https://dx.doi.org/10.1038/ncomms8452

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Hodak H, Clantin B, Willery E, Villeret V, Locht C, Jacob-Dubuisson F. Secretion signal of the filamentous haemagglutinin, a model two-partner secretion substrate. Mol Microbiol. 2006 Jul;61(2):368-82. Epub 2006 Jun 12. PMID:16771844 doi:10.1111/j.1365-2958.2006.05242.x

[2] Maier T, Clantin B, Gruss F, Dewitte F, Delattre AS, Jacob-Dubuisson F, Hiller S, Villeret V. Conserved Omp85 lid-lock structure and substrate recognition in FhaC. Nat Commun. 2015 Jun 10;6:7452. doi: 10.1038/ncomms8452. PMID:26058369 doi:https://dx.doi.org/10.1038/ncomms8452

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='4ql0' size='340' side='right'caption='[[4ql0]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ql0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borpe Borpe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ql0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis_Tohama_I Bordetella pertussis Tohama I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qky|4qky]], [[2qdz|2qdz]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql0 OCA], [https://pdbe.org/4ql0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql0 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql0 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BP1884, fhaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=257313 BORPE])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql0 OCA], [http://pdbe.org/4ql0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ql0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FHAC_BORPE FHAC_BORPE]] Member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin (FHA).<ref>PMID:16771844</ref>
+[https://www.uniprot.org/uniprot/FHAC_BORPE FHAC_BORPE] Member of a two partner secretion pathway (TPS) in which it mediates the secretion of filamentous hemagglutinin (FHA).<ref>PMID:16771844</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Borpe]]
+[[Category: Bordetella pertussis Tohama I]]
 [[Category: Large Structures]]
-[[Category: Clantin, B]]
+[[Category: Clantin B]]
-[[Category: Delattre, A S]]
+[[Category: Delattre AS]]
-[[Category: Dewitte, F]]
+[[Category: Dewitte F]]
-[[Category: Gruss, F]]
+[[Category: Gruss F]]
-[[Category: Hiller, S]]
+[[Category: Hiller S]]
-[[Category: Jacob-Dubuisson, F]]
+[[Category: Jacob-Dubuisson F]]
-[[Category: Maier, T]]
+[[Category: Maier T]]
-[[Category: Villeret, V]]
+[[Category: Villeret V]]
-[[Category: Beta-barrel]]
-[[Category: Outer membrane]]
-[[Category: Potra domain]]
-[[Category: Protein transport]]

4ql0: Difference between revisions

Revision as of 11:34, 15 February 2023

Crystal Structure Analysis of the Membrane Transporter FhaC (double mutant V169T, I176N)

Structural highlights

Function

Publication Abstract from PubMed

References

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