2luy: Difference between revisions

Revision as of 13:11, 22 February 2023

Solution structure of the tandem zinc finger domain of fission yeast Stc1

Structural highlights

2luy is a 1 chain structure with sequence from Schizosaccharomyces pombe 972h-. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

YORS_SCHPO Required for meiotic chromosome segregation.^[1]

Publication Abstract from PubMed

Noncoding RNAs can modulate gene expression by directing modifications to histones that alter chromatin structure. In fission yeast, siRNAs produced via the RNAi pathway direct modifications associated with heterochromatin formation. siRNAs associate with the RNAi effector protein Argonaute 1 (Ago1), targeting the Ago1-containing RNA-induced transcriptional silencing (RITS) complex to homologous nascent transcripts. This promotes recruitment of the Clr4 complex (CLRC), which mediates methylation of histone H3 on lysine 9 (H3K9me) in cognate chromatin. A key question is how the RNAi and chromatin modification machineries are connected. Stc1 is a small protein recently shown to associate with both Ago1 and CLRC and to play a pivotal role in mediating the RNAi-dependent recruitment of CLRC to chromatin. To understand its mode of action, we have performed a detailed structural and functional analysis of the Stc1 protein. Our analyses reveal that the conserved N-terminal region of Stc1 represents an unusual tandem zinc finger domain, with similarities to common LIM domains but distinguished by a lack of preferred relative orientation of the two zinc fingers. We demonstrate that this tandem zinc finger domain is involved in binding Ago1, whereas the nonconserved C-terminal region mediates association with CLRC. These findings elucidate the molecular basis for the coupling of RNAi to chromatin modification in fission yeast.

Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification.,He C, Pillai SS, Taglini F, Li F, Ruan K, Zhang J, Wu J, Shi Y, Bayne EH Proc Natl Acad Sci U S A. 2013 Apr 23. PMID:23613586^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gregan J, Rabitsch PK, Sakem B, Csutak O, Latypov V, Lehmann E, Kohli J, Nasmyth K. Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast. Curr Biol. 2005 Sep 20;15(18):1663-9. PMID:16169489 doi:https://dx.doi.org/10.1016/j.cub.2005.07.059
↑ He C, Pillai SS, Taglini F, Li F, Ruan K, Zhang J, Wu J, Shi Y, Bayne EH. Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification. Proc Natl Acad Sci U S A. 2013 Apr 23. PMID:23613586 doi:10.1073/pnas.1212155110

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OCA

[1] Gregan J, Rabitsch PK, Sakem B, Csutak O, Latypov V, Lehmann E, Kohli J, Nasmyth K. Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast. Curr Biol. 2005 Sep 20;15(18):1663-9. PMID:16169489 doi:https://dx.doi.org/10.1016/j.cub.2005.07.059

[2] He C, Pillai SS, Taglini F, Li F, Ruan K, Zhang J, Wu J, Shi Y, Bayne EH. Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification. Proc Natl Acad Sci U S A. 2013 Apr 23. PMID:23613586 doi:10.1073/pnas.1212155110

[1]

[2]

@@ Line 1: / Line 1: @@
 ==Solution structure of the tandem zinc finger domain of fission yeast Stc1==
-<StructureSection load='2luy' size='340' side='right'caption='[[2luy]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2luy' size='340' side='right'caption='[[2luy]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2luy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2luy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LUY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LUY FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPBP8B7.28c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2luy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2luy OCA], [https://pdbe.org/2luy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2luy RCSB], [https://www.ebi.ac.uk/pdbsum/2luy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2luy ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/YORS_SCHPO YORS_SCHPO]] Required for meiotic chromosome segregation.<ref>PMID:16169489</ref>
+[https://www.uniprot.org/uniprot/YORS_SCHPO YORS_SCHPO] Required for meiotic chromosome segregation.<ref>PMID:16169489</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Fission yeast]]
 [[Category: Large Structures]]
-[[Category: Bayne, E]]
+[[Category: Schizosaccharomyces pombe 972h-]]
-[[Category: He, C]]
+[[Category: Bayne E]]
-[[Category: Shi, Y]]
+[[Category: He C]]
-[[Category: Wu, J]]
+[[Category: Shi Y]]
-[[Category: Dna binding protein]]
+[[Category: Wu J]]
-[[Category: Tandem zinc finger]]

2luy: Difference between revisions

Revision as of 13:11, 22 February 2023

Solution structure of the tandem zinc finger domain of fission yeast Stc1

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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