2mgz: Difference between revisions

Revision as of 06:35, 2 March 2023

Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in ternary complex with RNA

Structural highlights

2mgz is a 3 chain structure with sequence from Caenorhabditis elegans. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G5EEW7_CAEEL

Publication Abstract from PubMed

Tissue-specific alternative pre-mRNA splicing is often cooperatively regulated by multiple splicing factors, but the structural basis of cooperative RNA recognition is poorly understood. In Caenorhabditis elegans, ligand binding specificity of fibroblast growth factor receptors (FGFRs) is determined by mutually exclusive alternative splicing of the sole FGFR gene, egl-15. Here we determined the solution structure of a ternary complex of the RNA-recognition motif (RRM) domains from the RBFOX protein ASD-1, SUP-12 and their target RNA from egl-15. The two RRM domains cooperatively interact with the RNA by sandwiching a G base to form the stable complex. Multichromatic fluorescence splicing reporters confirmed the requirement of the G and the juxtaposition of the respective cis elements for effective splicing regulation in vivo. Moreover, we identified a new target for the heterologous complex through an element search, confirming the functional significance of the intermolecular coordination.

RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing.,Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Guntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y Nat Struct Mol Biol. 2014 Sep;21(9):778-86. doi: 10.1038/nsmb.2870. Epub 2014 Aug, 17. PMID:25132178^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Guntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y. RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing. Nat Struct Mol Biol. 2014 Sep;21(9):778-86. doi: 10.1038/nsmb.2870. Epub 2014 Aug, 17. PMID:25132178 doi:https://dx.doi.org/10.1038/nsmb.2870

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Guntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y. RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing. Nat Struct Mol Biol. 2014 Sep;21(9):778-86. doi: 10.1038/nsmb.2870. Epub 2014 Aug, 17. PMID:25132178 doi:https://dx.doi.org/10.1038/nsmb.2870

[1]

@@ Line 1: / Line 1: @@
 ==Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in ternary complex with RNA==
-<StructureSection load='2mgz' size='340' side='right'caption='[[2mgz]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2mgz' size='340' side='right'caption='[[2mgz]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mgz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Caeel Caeel]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MGZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mgz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MGZ FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ru3|2ru3]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mgz OCA], [https://pdbe.org/2mgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mgz RCSB], [https://www.ebi.ac.uk/pdbsum/2mgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mgz ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">asd-1, CELE_R74.5, R74.5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), sup-12, CELE_T22B2.4, T22B2.4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mgz OCA], [https://pdbe.org/2mgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mgz RCSB], [https://www.ebi.ac.uk/pdbsum/2mgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mgz ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/G5EEW7_CAEEL G5EEW7_CAEEL]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Caeel]]
+[[Category: Caenorhabditis elegans]]
 [[Category: Large Structures]]
-[[Category: Guntert, P]]
+[[Category: Guntert P]]
-[[Category: Hagiwara, M]]
+[[Category: Hagiwara M]]
-[[Category: He, F]]
+[[Category: He F]]
-[[Category: Ito, T]]
+[[Category: Ito T]]
-[[Category: Kobayashi, N]]
+[[Category: Kobayashi N]]
-[[Category: Kuroyanagi, H]]
+[[Category: Kuroyanagi H]]
-[[Category: Kuwasako, K]]
+[[Category: Kuwasako K]]
-[[Category: Muto, Y]]
+[[Category: Muto Y]]
-[[Category: Shirouzu, M]]
+[[Category: Shirouzu M]]
-[[Category: Takahashi, M]]
+[[Category: Takahashi M]]
-[[Category: Tanaka, A]]
+[[Category: Tanaka A]]
-[[Category: Tsuda, K]]
+[[Category: Tsuda K]]
-[[Category: Unzai, S]]
+[[Category: Unzai S]]
-[[Category: Yokoyama, S]]
+[[Category: Yokoyama S]]
-[[Category: Yoshikawa, S]]
+[[Category: Yoshikawa S]]
-[[Category: Protein-rna complex]]
-[[Category: Rna binding protein-rna complex]]
-[[Category: Solution structure]]
-[[Category: Ternary complex]]

2mgz: Difference between revisions

Revision as of 06:35, 2 March 2023

Solution structure of RBFOX family ASD-1 RRM and SUP-12 RRM in ternary complex with RNA

Structural highlights

Function

Publication Abstract from PubMed

References

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