4rb2

Crystal structure of Magnetospirillum gryphiswaldense MSR-1 SeMet-Fur-Mn2+-feoAB1 operator

spinqualitylabelsall models 4rb2, resolution 2.82Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4rb2 is a 4 chain structure with sequence from Magnetospirillum gryphiswaldense MSR-1 v2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MN, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function V6F4Q0_MAGGM Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes.[ARBA:ARBA00002997] Publication Abstract from PubMed Ferric uptake regulator (Fur) plays a key role in the iron homeostasis of prokaryotes, such as bacterial pathogens, but the molecular mechanisms and structural basis of Fur-DNA binding remain incompletely understood. Here, we report high-resolution structures of Magnetospirillum gryphiswaldense MSR-1 Fur in four different states: apo-Fur, holo-Fur, the Fur-feoAB1 operator complex and the Fur-Pseudomonas aeruginosa Fur box complex. Apo-Fur is a transition metal ion-independent dimer whose binding induces profound conformational changes and confers DNA-binding ability. Structural characterization, mutagenesis, biochemistry and in vivo data reveal that Fur recognizes DNA by using a combination of base readout through direct contacts in the major groove and shape readout through recognition of the minor-groove electrostatic potential by lysine. The resulting conformational plasticity enables Fur binding to diverse substrates. Our results provide insights into metal ion activation and substrate recognition by Fur that suggest pathways to engineer magnetotactic bacteria and antipathogenic drugs. Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator.,Deng Z, Wang Q, Liu Z, Zhang M, Machado AC, Chiu TP, Feng C, Zhang Q, Yu L, Qi L, Zheng J, Wang X, Huo X, Qi X, Li X, Wu W, Rohs R, Li Y, Chen Z Nat Commun. 2015 Jul 2;6:7642. doi: 10.1038/ncomms8642. PMID:26134419 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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