5k5g: Difference between revisions

Revision as of 07:39, 8 March 2023

Structure of human islet amyloid polypeptide in complex with an engineered binding protein

Structural highlights

5k5g is a 3 chain structure with sequence from Homo sapiens and Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IAPP_HUMAN Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.

Publication Abstract from PubMed

In type 2 diabetes, the formation of islet amyloid consisting of islet amyloid polypeptide (IAPP) is associated with reduction in beta-cell mass and contributes to the failure of islet cell transplantation. Rational design of inhibitors of IAPP amyloid formation has therapeutic potential, but is hampered by the lack of structural information on inhibitor complexes of the conformationally flexible, aggregation-prone IAPP. Here we characterize a beta-hairpin conformation of IAPP in complex with the engineered binding protein beta-wrapin HI18. The beta-strands correspond to two amyloidogenic motifs, 12-LANFLVH-18 and 22-NFGAILS-28, which are connected by a turn established around Ser-20. Besides backbone hydrogen bonding, the IAPP:HI18 interaction surface is dominated by non-polar contacts involving hydrophobic side chains of the IAPP beta-strands. Apart from monomers, HI18 binds oligomers and fibrils and inhibits IAPP aggregation and toxicity at low substoichiometric concentrations. The IAPP beta-hairpin can serve as a molecular recognition motif enabling control of IAPP aggregation.

beta-Hairpin of Islet Amyloid Polypeptide Bound to an Aggregation Inhibitor.,Mirecka EA, Feuerstein S, Gremer L, Schroder GF, Stoldt M, Willbold D, Hoyer W Sci Rep. 2016 Sep 19;6:33474. doi: 10.1038/srep33474. PMID:27641459^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mirecka EA, Feuerstein S, Gremer L, Schroder GF, Stoldt M, Willbold D, Hoyer W. beta-Hairpin of Islet Amyloid Polypeptide Bound to an Aggregation Inhibitor. Sci Rep. 2016 Sep 19;6:33474. doi: 10.1038/srep33474. PMID:27641459 doi:https://dx.doi.org/10.1038/srep33474

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Mirecka EA, Feuerstein S, Gremer L, Schroder GF, Stoldt M, Willbold D, Hoyer W. beta-Hairpin of Islet Amyloid Polypeptide Bound to an Aggregation Inhibitor. Sci Rep. 2016 Sep 19;6:33474. doi: 10.1038/srep33474. PMID:27641459 doi:https://dx.doi.org/10.1038/srep33474

[1]

@@ Line 1: / Line 1: @@
 ==Structure of human islet amyloid polypeptide in complex with an engineered binding protein==
-<StructureSection load='5k5g' size='340' side='right'caption='[[5k5g]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='5k5g' size='340' side='right'caption='[[5k5g]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5k5g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K5G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5k5g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K5G FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IAPP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5g OCA], [https://pdbe.org/5k5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k5g RCSB], [https://www.ebi.ac.uk/pdbsum/5k5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5g ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k5g OCA], [https://pdbe.org/5k5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k5g RCSB], [https://www.ebi.ac.uk/pdbsum/5k5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k5g ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/IAPP_HUMAN IAPP_HUMAN]] Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
+[https://www.uniprot.org/uniprot/IAPP_HUMAN IAPP_HUMAN] Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Synthetic construct sequences]]
+[[Category: Synthetic construct]]
-[[Category: Feuerstein, S]]
+[[Category: Feuerstein S]]
-[[Category: Gremer, L]]
+[[Category: Gremer L]]
-[[Category: Hoyer, W]]
+[[Category: Hoyer W]]
-[[Category: Mirecka, E A]]
+[[Category: Mirecka EA]]
-[[Category: Schroeder, G F]]
+[[Category: Schroeder GF]]
-[[Category: Stoldt, M]]
+[[Category: Stoldt M]]
-[[Category: Willbold, D]]
+[[Category: Willbold D]]
-[[Category: Amyloid]]
-[[Category: Beta-hairpin]]
-[[Category: Hormone]]
-[[Category: Signaling protein]]
-[[Category: Type 2 diabetes]]

5k5g: Difference between revisions

Revision as of 07:39, 8 March 2023

Structure of human islet amyloid polypeptide in complex with an engineered binding protein

Structural highlights

Function

Publication Abstract from PubMed

References

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