2mkc: Difference between revisions

Revision as of 07:59, 8 March 2023

Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

Structural highlights

2mkc is a 3 chain structure with sequence from Saccharomyces cerevisiae S288C. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IST3_YEAST Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.^[1] ^[2] ^[3]

Publication Abstract from PubMed

The precursor mRNA (pre-mRNA) retention and splicing (RES) complex is a spliceosomal complex that is present in yeast and humans and is important for RNA splicing and retention of unspliced pre-mRNA. Here, we present the solution NMR structure of the RES core complex from Saccharomyces cerevisiae. Complex formation leads to an intricate folding of three components-Snu17p, Bud13p and Pml1p-that stabilizes the RNA-recognition motif (RRM) fold of Snu17p and increases binding affinity in tertiary interactions between the components by more than 100-fold compared to that in binary interactions. RES interacts with pre-mRNA within the spliceosome, and through the assembly of the RES core complex RNA binding efficiency is increased. The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome.

Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.,Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gottschalk A, Bartels C, Neubauer G, Luhrmann R, Fabrizio P. A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol. 2001 May;21(9):3037-46. PMID:11287609 doi:https://dx.doi.org/10.1128/MCB.21.9.3037-3046.2001
↑ Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprevote O, Seraphin B. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25. PMID:15565172 doi:https://dx.doi.org/7600482
↑ Spingola M, Armisen J, Ares M Jr. Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p. Nucleic Acids Res. 2004 Feb 18;32(3):1242-50. Print 2004. PMID:14973223 doi:https://dx.doi.org/10.1093/nar/gkh281
↑ Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M. Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446 doi:https://dx.doi.org/10.1038/nsmb.2889

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Gottschalk A, Bartels C, Neubauer G, Luhrmann R, Fabrizio P. A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol. 2001 May;21(9):3037-46. PMID:11287609 doi:https://dx.doi.org/10.1128/MCB.21.9.3037-3046.2001

[2] Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprevote O, Seraphin B. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25. PMID:15565172 doi:https://dx.doi.org/7600482

[3] Spingola M, Armisen J, Ares M Jr. Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p. Nucleic Acids Res. 2004 Feb 18;32(3):1242-50. Print 2004. PMID:14973223 doi:https://dx.doi.org/10.1093/nar/gkh281

[4] Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M. Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446 doi:https://dx.doi.org/10.1038/nsmb.2889

[1]

[2]

[3]

[4]

@@ Line 1: / Line 1: @@
 ==Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex==
-<StructureSection load='2mkc' size='340' side='right'caption='[[2mkc]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2mkc' size='340' side='right'caption='[[2mkc]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mkc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MKC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mkc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MKC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MKC FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IST3, SNU17, YIB5W, YIR005W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), PML1, YLR016C, L1591 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), BUD13, CWC26, SLC7, YGL174W, G1642 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mkc OCA], [https://pdbe.org/2mkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mkc RCSB], [https://www.ebi.ac.uk/pdbsum/2mkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mkc ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mkc OCA], [https://pdbe.org/2mkc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mkc RCSB], [https://www.ebi.ac.uk/pdbsum/2mkc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mkc ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/IST3_YEAST IST3_YEAST]] Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.<ref>PMID:11287609</ref> <ref>PMID:15565172</ref> <ref>PMID:14973223</ref>  [[https://www.uniprot.org/uniprot/CWC26_YEAST CWC26_YEAST]] Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.<ref>PMID:8657162</ref> <ref>PMID:11452010</ref> <ref>PMID:12871902</ref> <ref>PMID:15565172</ref>  [[https://www.uniprot.org/uniprot/PML1_YEAST PML1_YEAST]] Required for efficient splicing and pre-mRNA nuclear retention.<ref>PMID:15565172</ref>
+[https://www.uniprot.org/uniprot/IST3_YEAST IST3_YEAST] Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.<ref>PMID:11287609</ref> <ref>PMID:15565172</ref> <ref>PMID:14973223</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Baker's yeast]]
 [[Category: Large Structures]]
-[[Category: Becker, S]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Luhrmann, R]]
+[[Category: Becker S]]
-[[Category: Munari, F]]
+[[Category: Luhrmann R]]
-[[Category: Schneider, C]]
+[[Category: Munari F]]
-[[Category: Trowitzsch, S]]
+[[Category: Schneider C]]
-[[Category: Wahl, M C]]
+[[Category: Trowitzsch S]]
-[[Category: Wysoczanski, P]]
+[[Category: Wahl MC]]
-[[Category: Xiang, S]]
+[[Category: Wysoczanski P]]
-[[Category: Zweckstetter, M]]
+[[Category: Xiang S]]
-[[Category: Bud13p]]
+[[Category: Zweckstetter M]]
-[[Category: Cooperativity]]
-[[Category: Heterotrimer]]
-[[Category: Ist3p]]
-[[Category: Pml1p]]
-[[Category: Protein binding]]
-[[Category: Re]]
-[[Category: Rrm]]
-[[Category: Snu17p]]
-[[Category: Spliceosome]]
-[[Category: Splicing]]

2mkc: Difference between revisions

Revision as of 07:59, 8 March 2023

Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools