8him: Difference between revisions
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==A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom== | |||
<StructureSection load='8him' size='340' side='right'caption='[[8him]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8him]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_oleracea Brassica oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HIM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8him OCA], [https://pdbe.org/8him PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8him RCSB], [https://www.ebi.ac.uk/pdbsum/8him PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8him ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0D2ZPP3_BRAOL A0A0D2ZPP3_BRAOL] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In addition to the conserved RNA polymerases (Pols) I-III in eukaryotes, two atypical polymerases, Pols IV and V, specifically produce non-coding RNA in the RNA-directed DNA methylation (RdDM) pathway in plants. Here, we report on the structures of cauliflower Pol V in the free and elongation conformations. A conserved tyrosine residue of NRPE2 stacks with a dsDNA branch of the transcription bubble to potentially attenuate elongation by inducing transcription stalling. The non-template DNA strand is captured by NRPE2 to enhance backtracking, thereby increasing 3'-5' cleavage which likely underpins Pol V's high fidelity. The structures also illuminate the mechanism of Pol V transcription stalling and enhanced backtracking which may be important for Pol V's retention on chromatin to serve its function in tethering downstream factors for RdDM. | |||
Structure and mechanism of the plant RNA polymerase V.,Xie G, Du X, Hu H, Li S, Cao X, Jacobsen SE, Du J Science. 2023 Mar 9:eadf8231. doi: 10.1126/science.adf8231. PMID:36893216<ref>PMID:36893216</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8him" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Brassica oleracea]] | |||
[[Category: Large Structures]] | |||
[[Category: Du J]] | |||
[[Category: Du X]] | |||
[[Category: Hu H]] | |||
[[Category: Xie G]] | |||
Revision as of 07:28, 22 March 2023
A cryo-EM structure of B. oleracea RNA polymerase V elongation complex at 2.73 Angstrom
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