4ui2: Difference between revisions

Revision as of 08:25, 22 March 2023

Crystal structure of the ternary RGMB-BMP2-NEO1 complex

Structural highlights

4ui2 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ACT, BMA, NAG, SRT
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NEO1_HUMAN May be involved as a regulatory protein in the transition of undifferentiated proliferating cells to their differentiated state. May also function as a cell adhesion molecule in a broad spectrum of embryonic and adult tissues.

Publication Abstract from PubMed

Repulsive guidance molecules (RGMs) control crucial processes including cell motility, adhesion, immune-cell regulation and systemic iron metabolism. RGMs signal via the neogenin (NEO1) and the bone morphogenetic protein (BMP) pathways. Here, we report crystal structures of the N-terminal domains of all human RGM family members in complex with the BMP ligand BMP2, revealing a new protein fold and a conserved BMP-binding mode. Our structural and functional data suggest a pH-linked mechanism for RGM-activated BMP signaling and offer a rationale for RGM mutations causing juvenile hemochromatosis. We also determined the crystal structure of the ternary BMP2-RGM-NEO1 complex, which, along with solution scattering and live-cell super-resolution fluorescence microscopy, indicates BMP-induced clustering of the RGM-NEO1 complex. Our results show how RGM acts as the central hub that links BMP and NEO1 and physically connects these fundamental signaling pathways.

Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein.,Healey EG, Bishop B, Elegheert J, Bell CH, Padilla-Parra S, Siebold C Nat Struct Mol Biol. 2015 May 4. doi: 10.1038/nsmb.3016. PMID:25938661^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Healey EG, Bishop B, Elegheert J, Bell CH, Padilla-Parra S, Siebold C. Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein. Nat Struct Mol Biol. 2015 May 4. doi: 10.1038/nsmb.3016. PMID:25938661 doi:https://dx.doi.org/10.1038/nsmb.3016

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OCA

[1] Healey EG, Bishop B, Elegheert J, Bell CH, Padilla-Parra S, Siebold C. Repulsive guidance molecule is a structural bridge between neogenin and bone morphogenetic protein. Nat Struct Mol Biol. 2015 May 4. doi: 10.1038/nsmb.3016. PMID:25938661 doi:https://dx.doi.org/10.1038/nsmb.3016

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='4ui2' size='340' side='right'caption='[[4ui2]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ui2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UI2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ui2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UI2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uhy|4uhy]], [[4uhz|4uhz]], [[4ui0|4ui0]], [[4ui1|4ui1]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ui2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ui2 OCA], [https://pdbe.org/4ui2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ui2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ui2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ui2 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ui2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ui2 OCA], [http://pdbe.org/4ui2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ui2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ui2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ui2 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RGMB_HUMAN RGMB_HUMAN]] Member of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system (By similarity). Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling (By similarity). Promotes neuronal adhesion (By similarity). May inhibit neurite outgrowth. [[http://www.uniprot.org/uniprot/NEO1_MOUSE NEO1_MOUSE]] May be involved as a regulatory protein in the transition of undifferentiated proliferating cells to their differentiated state. May also function as a cell adhesion molecule in a broad spectrum of embryonic and adult tissues. [[http://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN]] Induces cartilage and bone formation.
+[https://www.uniprot.org/uniprot/NEO1_HUMAN NEO1_HUMAN] May be involved as a regulatory protein in the transition of undifferentiated proliferating cells to their differentiated state. May also function as a cell adhesion molecule in a broad spectrum of embryonic and adult tissues.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Bell, C H]]
+[[Category: Bell CH]]
-[[Category: Bishop, B]]
+[[Category: Bishop B]]
-[[Category: Elegheert, J]]
+[[Category: Elegheert J]]
-[[Category: Healey, E G]]
+[[Category: Healey EG]]
-[[Category: Padilla-Parra, S]]
+[[Category: Padilla-Parra S]]
-[[Category: Siebold, C]]
+[[Category: Siebold C]]
-[[Category: Axon guidance]]
-[[Category: Bone morphogenetic protein pathway]]
-[[Category: Cell surface receptor signaling]]
-[[Category: Hemojuvelin]]
-[[Category: Morphogen]]
-[[Category: Neogenin]]
-[[Category: Repulsive guidance molecule]]
-[[Category: Signaling protein]]

4ui2: Difference between revisions

Revision as of 08:25, 22 March 2023

Crystal structure of the ternary RGMB-BMP2-NEO1 complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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