8g0p: Difference between revisions
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The | ==Crystal structure of the human Ndc80:Nuf2 loop region== | ||
<StructureSection load='8g0p' size='340' side='right'caption='[[8g0p]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8g0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G0P FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g0p OCA], [https://pdbe.org/8g0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g0p RCSB], [https://www.ebi.ac.uk/pdbsum/8g0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g0p ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The conserved Ndc80 kinetochore complex, Ndc80c, is the principal link between mitotic spindle microtubules and centromere-associated proteins. We used AlphaFold 2 (AF2) to obtain predictions of the Ndc80 'loop' structure and of the Ndc80 : Nuf2 globular head domains that interact with the Dam1 subunit of the heterodecameric DASH/Dam1 complex (Dam1c). The predictions guided design of crystallizable constructs, with structures close to the predicted ones. The Ndc80 'loop' is a stiff, alpha-helical 'switchback' structure; AF2 predictions and positions of preferential cleavage sites indicate that flexibility within the long Ndc80c rod occurs instead at a hinge closer to the globular head. Conserved stretches of the Dam1 C terminus bind Ndc80c such that phosphorylation of Dam1 serine residues 257, 265 and 292 by the mitotic kinase Ipl1/Aurora B can release this contact during error correction of mis-attached kinetochores. We integrate the structural results presented here into our current molecular model of the kinetochore-microtubule interface. The model illustrates how multiple interactions between Ndc80c, DASH/Dam1c and the microtubule lattice stabilize kinetochore attachments. | |||
Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.,Zahm JA, Jenni S, Harrison SC Open Biol. 2023 Mar;13(3):220378. doi: 10.1098/rsob.220378. Epub 2023 Mar 8. PMID:36883282<ref>PMID:36883282</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Harrison | <div class="pdbe-citations 8g0p" style="background-color:#fffaf0;"></div> | ||
[[Category: Jenni | == References == | ||
[[Category: Zahm | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Harrison SC]] | |||
[[Category: Jenni S]] | |||
[[Category: Zahm JA]] | |||
Revision as of 06:54, 29 March 2023
Crystal structure of the human Ndc80:Nuf2 loop region
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