4uri

Crystal structure of chitinase-like agglutinin RobpsCRA from Robinia pseudoacacia

spinqualitylabelsall models 4uri, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4uri is a 2 chain structure with sequence from Robinia pseudoacacia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, MPD, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A1YZD2_ROBPS Publication Abstract from PubMed A new chitinase-like agglutinin, RobpsCRA, related to family GH18 chitinases, has previously been identified in black locust (Robinia pseudoacacia) bark. The crystal structure of RobpsCRA at 1.85A resolution reveals unusual molecular determinants responsible for the lack of its ancestral chitinase activity. Unlike other chitinase-like proteins, which lack chitinase catalytic residues, RobpsCRA has conserved its catalytic machinery. However, concerted rearrangements of loop regions coupled to non-conservative substitutions of aromatic residues central to the chitin-binding groove explain the lack of hydrolytic activity against chitin and the switch toward recognition of high-mannose type N-glycans. Identification of close homologs in flowering plants with conservation of sequence motifs associated to the structural adaptations seen in RobpsCRA defines an emerging class of agglutinins, as emphasized by a phylogenetic analysis, that are likely to share a similar carbohydrate binding specificity for high-mannose type N-glycans. This study illustrates the recent evolution and molecular adaptation of a versatile TIM-barrel scaffold within the ancestral GH18 family. Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery.,Sulzenbacher G, Roig-Zamboni V, Peumans WJ, Henrissat B, van Damme EJ, Bourne Y J Struct Biol. 2015 Feb 26. pii: S1047-8477(15)00029-5. doi:, 10.1016/j.jsb.2015.01.013. PMID:25727185 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Agglutinin 3D structures

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