Sandbox Reserved 1783: Difference between revisions

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== Structural Overview ==

NTCP has 9 transmembrane alpha helices (TM) that form the protein, with an extracellular N-terminus. NTCP has two domains within the protein, <scene name='95/952711/Panel_domain/1'>a panel domain</scene>, made up of TM1, TM5, and TM6, and <scene name='95/952711/Core_domain/3'>a core domain</scene>, made up of TM2-4 and TM7-9. An interesting feature of NTCP is the cross of TM3 and TM8 that form an <scene name='95/952711/X_motif/1'>X motif</scene> within the protein. The two domains are essential to the conformation change of NTCP to bind bile salts. [https://www.sciencedirect.com/science/article/pii/S266667582200090X?via%3Dihub]

NTCP has 9 transmembrane alpha helices (TM) that form the protein, with an extracellular N-terminus and intracellular C-terminus. NTCP has two domains within the protein, <scene name='95/952711/Panel_domain/1'>a panel domain</scene>, made up of TM1, TM5, and TM6, and <scene name='95/952711/Core_domain/3'>a core domain</scene>, made up of TM2-4 and TM7-9. An interesting feature of NTCP is the cross of TM3 and TM8 that form an <scene name='95/952711/X_motif/1'>X motif</scene> within the protein. The two domains are essential to the conformation change of NTCP to bind bile salts. [https://www.sciencedirect.com/science/article/pii/S266667582200090X?via%3Dihub]

There are two significant areas in the NTCP structure that facilitate ligand binding, which are referred to as "patches." Residues 84-87 of NTCP are patch 1, which are located on the TM2-TM3 loop in the core domain. This is also considered the extracellular region of NTCP “tunnel." Residues 157-165 NTCP are associated with patch 2. They are located on N-terminal half of the TM5 in the panel domain (residue sequence: KGIVISLVL). Patch 2 is also located in th extracellular region. These residues' importance was determined through mutations of these residues and examined through pull-down assays (Asami, et. al, 2022).

Sandbox Reserved 1783: Difference between revisions

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@@ Line 11: / Line 11: @@
 == Structural Overview ==
-NTCP has 9 transmembrane alpha helices (TM) that form the protein, with an extracellular N-terminus. NTCP has two domains within the protein, <scene name='95/952711/Panel_domain/1'>a panel domain</scene>, made up of TM1, TM5, and TM6, and <scene name='95/952711/Core_domain/3'>a core domain</scene>, made up of TM2-4 and TM7-9. An interesting feature of NTCP is the cross of TM3 and TM8 that form an <scene name='95/952711/X_motif/1'>X motif</scene> within the protein. The two domains are essential to the conformation change of NTCP to bind bile salts. [https://www.sciencedirect.com/science/article/pii/S266667582200090X?via%3Dihub]
+NTCP has 9 transmembrane alpha helices (TM) that form the protein, with an extracellular N-terminus and intracellular C-terminus. NTCP has two domains within the protein, <scene name='95/952711/Panel_domain/1'>a panel domain</scene>, made up of TM1, TM5, and TM6, and <scene name='95/952711/Core_domain/3'>a core domain</scene>, made up of TM2-4 and TM7-9. An interesting feature of NTCP is the cross of TM3 and TM8 that form an <scene name='95/952711/X_motif/1'>X motif</scene> within the protein. The two domains are essential to the conformation change of NTCP to bind bile salts. [https://www.sciencedirect.com/science/article/pii/S266667582200090X?via%3Dihub]
 There are two significant areas in the NTCP structure that facilitate ligand binding, which are referred to as "patches." Residues 84-87 of NTCP are patch 1, which are located on the TM2-TM3 loop in the core domain. This is also considered the extracellular region of NTCP “tunnel." Residues 157-165 NTCP are associated with patch 2. They are located on N-terminal half of the TM5 in the panel domain (residue sequence: KGIVISLVL). Patch 2 is also located in th extracellular region. These residues' importance was determined through mutations of these residues and examined through pull-down assays (Asami, et. al, 2022).