BASIL2023GVQ8DN35: Difference between revisions
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== Protein purification analysis == | == Protein purification analysis == | ||
Through the use of a gravity purification column and Ni-NTA resin, Q8DN35 was purified and a Bradford analysis was conducted to determine the amount of protein in the purified sample. This purification resulted in 0.055 mg/mL of protein being purified. Through SDS-PAGE analysis, observation of purified protein was hard to see in the fraction column but large banding occurred in the pre- and post-induction samples around 31.88 kDa, which is the predicted molecular weight for Q8DN35. Further analysis with more concentrated protein sample will need to be done to confirm correct protein positioning. | Through the use of a gravity purification column and Ni-NTA resin, Q8DN35 was purified and a Bradford analysis was conducted to determine the amount of protein in the purified sample. This purification resulted in 0.055 mg/mL of protein being purified. Through SDS-PAGE analysis, observation of purified protein was hard to see in the fraction column but large banding occurred in the pre- and post-induction samples around 31.88 kDa, which is the predicted molecular weight for Q8DN35. Further analysis with more concentrated protein sample will need to be done to confirm correct protein positioning. | ||
[[Image:Q8DN35 initial SDS-PAGE.png]] | |||
== Kinase activity analysis == | == Kinase activity analysis == | ||
To observe Q8DN35's activity, a kinase activity was ran with 2 substrates to observe potential substrate usage. The 2 substrates that were tested are NAG and F6P. | To observe Q8DN35's activity, a kinase activity was ran with 2 substrates to observe potential substrate usage. The 2 substrates that were tested are NAG and F6P. | ||
