BASIL2023GVQ8DN35: Difference between revisions
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To observe Q8DN35's activity, a kinase activity was ran with 2 substrates to observe potential substrate usage. The 2 substrates that were tested are NAG and F6P. After kinetics calculations were finished, we conclude that NAG has a negative effect on Q8DN35 activity and F6P has a positive effect. The magnitude of increase in activity was not ideal, so further exploration into substrates and kinetic assay protocols will need to be done to determine the correct substrate for Q8DN35. | To observe Q8DN35's activity, a kinase activity was ran with 2 substrates to observe potential substrate usage. The 2 substrates that were tested are NAG and F6P. After kinetics calculations were finished, we conclude that NAG has a negative effect on Q8DN35 activity and F6P has a positive effect. The magnitude of increase in activity was not ideal, so further exploration into substrates and kinetic assay protocols will need to be done to determine the correct substrate for Q8DN35. | ||
[[Image:Kinase assay table for Q8DN35.JPG]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
