Sandbox Reserved 1785: Difference between revisions
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Due to the poor resolution of the Fab region, specific side chain interactions between the heavy ('''{{Font color|violet|A}}'''/<b><span class="text-blue">B</span></b>) and light (<b><span class="text-purple">A</span></b>/<b><span class="text-cyan">B</span></b>) chains have not been determined. It is estimated that each β-sandwich contains one disulfide bridge with additional hydrogen bonds. The <scene name='95/952713/Heavy-light_chain_interface/2'>heavy-light chain interface</scene> shows how the four heavy and light chain β-sandwiches fit together. The Fab region heavy chains attach to the Fc region heavy chains, before continuing down into the intracellular domain to interact with the <b><span class="text-brown">Igα</span></b>/<b><span class="text-orange">Igβ</span></b> subunits. The light chains (<b><span class="text-purple">A</span></b>/<b><span class="text-cyan">B</span></b>) however are only connected to the heavy chains ('''{{Font color|violet|A}}'''/<b><span class="text-blue">B</span></b>) within the Fab region, thus have no contact with the <b><span class="text-brown">Igα</span></b>/<b><span class="text-orange">Igβ</span></b> heterodimer. | Due to the poor resolution of the Fab region, specific side chain interactions between the heavy ('''{{Font color|violet|A}}'''/<b><span class="text-blue">B</span></b>) and light (<b><span class="text-purple">A</span></b>/<b><span class="text-cyan">B</span></b>) chains have not been determined. It is estimated that each β-sandwich contains one disulfide bridge with additional hydrogen bonds. The <scene name='95/952713/Heavy-light_chain_interface/2'>heavy-light chain interface</scene> shows how the four heavy and light chain β-sandwiches fit together. The Fab region heavy chains attach to the Fc region heavy chains, before continuing down into the intracellular domain to interact with the <b><span class="text-brown">Igα</span></b>/<b><span class="text-orange">Igβ</span></b> subunits. The light chains (<b><span class="text-purple">A</span></b>/<b><span class="text-cyan">B</span></b>) however are only connected to the heavy chains ('''{{Font color|violet|A}}'''/<b><span class="text-blue">B</span></b>) within the Fab region, thus have no contact with the <b><span class="text-brown">Igα</span></b>/<b><span class="text-orange">Igβ</span></b> heterodimer. | ||
[[Image: | [[Image:IgM_Surface2.png|400 px|left|thumb|'''Figure 3. Surface Representation of IgM Antibody Binding Pocket.''' On one arm of the IgM antibody, the antigen makes contact with light chain A at the L1 and L3 complementary-determining regions. Furthermore, it makes contact with heavy chain A at the H1, H2, and H3 complementary-determining regions. The location of the complementary-determining regions were approximated using the structure of the VCR01 variable region and were visualized using Pymol.]] | ||
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