Sandbox Reserved 1777: Difference between revisions
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NTpS can bind to the PP1C active site without PP1C also being bound to SHOC2 and MRAS, however, the catalytic activity is much slower and the reaction is less efficient. In order for NTpS to bind PP1C in the absence of the other two proteins, NTpS would need to be exposed from its binding site in the inactive RAF complex. RAS has to bind to RAF to expose the NTpS allowing PP1C and NTpS to bind. | NTpS can bind to the PP1C active site without PP1C also being bound to SHOC2 and MRAS, however, the catalytic activity is much slower and the reaction is less efficient. In order for NTpS to bind PP1C in the absence of the other two proteins, NTpS would need to be exposed from its binding site in the inactive RAF complex. RAS has to bind to RAF to expose the NTpS allowing PP1C and NTpS to bind. | ||
<scene name='95/952705/Pp1c_active_site/5'>TextToBeDisplayed</scene> '''let me know thought on the style of image''' | <scene name='95/952705/Pp1c_active_site/5'>TextToBeDisplayed</scene> '''let me know thought on the style of image''' | ||
=Activation of RAF= | =Activation of RAF= | ||
[[Image:Screen Shot 2023-04-10 at 4.34.56 PM.jpeg|600px|thumb|<font size="2"><div style="text-align: center;">'''Figure 3'''. Schematic representation of RAF activation. | [[Image:Screen Shot 2023-04-10 at 4.34.56 PM.jpeg|600px|thumb|<font size="2"><div style="text-align: center;">'''Figure 3'''. Schematic representation of RAF activation. PP1C removes an inhibitory phosphate group from RAF, which results in RAF activation and activation of the RAF/MAPK signaling cascade. </div></font>]] | ||
Once the SMP complex comes together, it plays a key role in regulating the activation of the RAF | Once the SMP complex comes together, it plays a key role in regulating the activation of the RAF/MAPK pathway. PP1C, enhanced through interactions with SHOC2 and MRAS, removes a phosphate group from serine residue 259 (also known as NTpS) of RAF. The removal of the phosphate group triggers activation of RAF, a serine/threonine kinase, which leads to the phosphorylation and activation of MAPK. This causes the activation of downstream proteins ERK1 and ERK2, which are responsible for activating nuclear transcription factors such as Elk-1, c-Ets1, C-Ets2, and MNK1, which result in the transcription of genes that code for proteins which promote cell proliferation and differentiation. <Ref name='Lavoie'>Lavoie, H., Therrien, M. Structural keys unlock RAS–MAPK cellular signaling pathway. Nature 609, 248-249 (2022). doi: 10.1038/d41586-022-02189-7. [https://doi.org/10.1038/d41586-022-02189-7. DOI:10.1038/d41586-022-02189-7]. </Ref>. | ||
=Implications= | =Implications= | ||
