Sandbox Reserved 1792: Difference between revisions

[[Image:Untitled (1).jpg|300 px|right|thumb| Figure 2: An overview of the Inactive (pink) vs Active (green) state of TSHR embedded in the plasma membrane. In the inactive state, the LRRD is pointed down. When TSH binds to the LRRD, confirmation changes in the Hinge Region and steric clashes between TSH and the Cell membrane cause TSHR to rotate into the active state. As shown, the LRRD rotates 55° into an upright position <ref name="Faust"/>. PDB: [[https://www.rcsb.org/structure/7T9M 7t9m]] and [https://www.rcsb.org/structure/7T9I 7t9i]]] [[Image:Inactive v active residue.png|300 px|right|thumb| Figure 3: A zoomed in view of the Y279 residue in the Hinge Region of TSHR, showing the 6Å rearrangement of Y279 during the activation of TSHR. Active TSHR is shown in green (PDB: [https://www.rcsb.org/structure/7T9I 7t9i]) and inactive TSHR is shown in pink (PDB: [https://www.rcsb.org/structure/7T9M 7t9m]).]]

In its restinf state without TSH bound, TSHR is in the <scene name='95/952720/Inactivetshr/8'>inactive state</scene>, also known as the "down" state because the LRRD is pointing down. When TSH binds to TSHR, steric clashes between TSH and the cell-membrane cause TSHR to take on the <scene name='95/952720/Inactivetshr/6'>active or "up" state</scene> (fig 2). During this transition, the LRRD rotate 55° along an axis perpendicular to the cell membrane. This rotation is initiated by conformational changes within the <scene name='95/952720/Hinge_region_spin/1'>Hinge Region</scene>, specifically at the <scene name='95/952720/Hinge_region_residues/2'>Tyr279 residue</scene>, located in the Hinge Region. Y279 moves 6 Å relative to I486, which is a residue located in the Transmembrane Region nearby Y279<ref name="Faust"/> (Fig 3).