Sandbox Reserved 1808

This Sandbox is Reserved from Mar 1 through Jun 1, 2023 for use in the course CHEM 351 Biochemistry taught by Bonnie_Hall at the Grand View University, Des Moines, USA. This reservation includes Sandbox Reserved 1796 through Sandbox Reserved 1811.

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Lactonase Enzyme Structure

spinqualitylabelsall models Caption for this structure Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D This is a default text for your page '. Click above on edit this page' to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia or to the article describing Jmol to the rescue. Function of Lactonase Enzyme The organism from which this protein is found is Rhodopsuedomonas plaustris (Rpa3624). The basic function for the lactonase enzyme is to break down valerolactone. Biological Relevance and Broader Implications Lactones are a diverse set of cyclic ester-containing compounds that play many roles in chemical and biological systems. Naturally occurring lactones function as metabolic intermediates, signaling molecules, antibiotics, and more. GVL is one such lactone used as a solvent that can be both produced from plant biomass and used to dissolve plant cell walls into sugars and aromatic molecules for subsequent microbial conversion to fuels and chemicals. Even after the majority is removed from the dissolved biomass, the residual GVL can be toxic to microbial fermentation. Residual GVL could also represent a significant carbon and energy source for tolerant microbes that could assimilate this lactone or convert it into other valuable products. The use of GVL as a renewable solvent for plant deconstruction would benefit from the identification of microbial enzymes that can hydrolyze the lactone ring of this compound. Important Amino Acids The first five amino acids of the amino terminus at physiological pH are MSNVR. Structural Highlights Secondary Structure: Consists of six blades of B-propeller, phosphate molecule bound to calcium and sodium ion with a helix attached to it. Tertiary Structure: Calcium coordination sphere shows the contacts holding the calcium ion in place. Quaternary structure: Carbonyl oxygen of 2-HQ overlaps with one of the phosphate oxygens.