Sandbox Reserved 1802: Difference between revisions
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== Function of your protein == | == Function of your protein == | ||
My protein is found in the Streptomyces coelicolor, but being expressed system in Escherichia coli. The specific function of Chorismate dehydratase is to catalyze the dehydration of chorismate to produce 3-enolpyruvyl-benzoate (3-EPB). Chorismate dehydratase has two ligands, both are (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid (ISJ). The two ISJ molecules are on different of lobes of Chorismate dehydratase (Lobe A and Lobe B). | My protein is found in the Streptomyces coelicolor, but being expressed system in Escherichia coli. The specific function of Chorismate dehydratase is to catalyze the dehydration of chorismate to produce 3-enolpyruvyl-benzoate (3-EPB). Chorismate dehydratase has two ligands, both are (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid (ISJ). The two ISJ molecules are on different of lobes of Chorismate dehydratase (Lobe A and Lobe B). | ||
== Biological relevance and broader implications == | == Biological relevance and broader implications == | ||
Not much is known about the futalosine pathway and the way it works. MqnA is the first enzyme of the futalosine pathway catalyzing the dehydration of chorismate to yield 3-enolpyruvyl-benzoate (3-EPB), a reaction not observed for any other enzyme. Chorismate is the product of the shikimate pathway and the precursor of many aromatic compounds including the amino acids in addition to the electron carriers ubiquinone and menaquinone. As enzymes of the shikimate pathway, as well as enzymes that convert chorismate, are only present in bacteria, fungi, and plants, they are promising targets for antimicrobials and herbicides. | Not much is known about the futalosine pathway and the way it works. MqnA is the first enzyme of the futalosine pathway catalyzing the dehydration of chorismate to yield 3-enolpyruvyl-benzoate (3-EPB), a reaction not observed for any other enzyme. Chorismate is the product of the shikimate pathway and the precursor of many aromatic compounds including the amino acids in addition to the electron carriers ubiquinone and menaquinone. As enzymes of the shikimate pathway, as well as enzymes that convert chorismate, are only present in bacteria, fungi, and plants, they are promising targets for antimicrobials and herbicides. | ||
== Important amino acids== | == Important amino acids== | ||
The catalytic amino acids in my enzyme are ALA17, SER86, and PHE243. These three amino acids would be <scene name='95/954099/Triad/1'>catalytic triad.</scene> | The catalytic amino acids in my enzyme are ALA17, SER86, and PHE243. These three amino acids would be <scene name='95/954099/Triad/1'>catalytic triad.</scene> | ||
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ISJ (B) has the following Hydrogen bonds with ScMqnA: THR59, SER86, SER109, ARG110, SER112, GLY151, PHE186, PHE243 | ISJ (B) has the following Hydrogen bonds with ScMqnA: THR59, SER86, SER109, ARG110, SER112, GLY151, PHE186, PHE243 | ||
ISJ (B) has the following Hydrophobic interaction with ScMqnA: PRO41, VAL78, VAL84, ILE150, PHE186 | ISJ (B) has the following Hydrophobic interaction with ScMqnA: PRO41, VAL78, VAL84, ILE150, PHE186 | ||
== Structural highlights == | == Structural highlights == | ||
The Secondary structures has 20 beta sheets, 28 alpha helices, and several random coils. The tertiary structure would be described as globular as is it in a shape of a ball, with an alpha clamp and two lobes connected by <scene name='95/954099/Structural_highlights/1'>two linkers</scene>. The beta sheets contain all three of the catalytic amino acids and most of the important binding amino acids. | The Secondary structures has 20 beta sheets, 28 alpha helices, and several random coils. The tertiary structure would be described as globular as is it in a shape of a ball, with an alpha clamp and two lobes connected by <scene name='95/954099/Structural_highlights/1'>two linkers</scene>. The beta sheets contain all three of the catalytic amino acids and most of the important binding amino acids. | ||
== Other Important Features == | == Other Important Features == | ||
