Sandbox Reserved 1799

This Sandbox is Reserved from Mar 1 through Jun 1, 2023 for use in the course CHEM 351 Biochemistry taught by Bonnie_Hall at the Grand View University, Des Moines, USA. This reservation includes Sandbox Reserved 1796 through Sandbox Reserved 1811.

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Pyrrolysyl-tRNA synthase (PylRS)

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Function of your protein

Pyrrolysyl-tRNA synthase (PylRS) is a protein found in anaerobic archaea and bacteria. PylRS facilitates the installation of pyrrolysine. It binds to the pyrrolysine substrate. This protein has two functional domains. It has a C-terminus, which is the catalytic domain (PylSc) and has a Rossmann fold, which is a tertiary fold that binds to nucleotides. The other functional domain is the N-terminus that does the tRNA binding (PylSn). These two functional domains can work together to install two noncanonical amino acids at the same time.

There are three types of ligands in this protein, YLY, POP (Pyrophosphate 2-) and EDO (1,2-ethanediol).

Important amino acids

Important amino acids highlighted.

Structural highlights

PylRS has two domains, an N-terminal and a C-terminal. The N-terminal is responsible for tRNA-binding, PylSn. The C-terminal is the catalytic domain, PylSc.